Daley M, Knauf V C, Summerfelt K R, Turner J C
Calgene Inc., 1920 Fifth St., Davis, CA 95616, USA, , , , , , US.
Plant Cell Rep. 1998 Apr;17(6-7):489-496. doi: 10.1007/s002990050430.
Co-transformation was investigated as a method that would allow the use of a selectable marker during plant regeneration followed by recovery of progeny which contain the desired gene(s) but lack a marker gene. Rapeseed (Brassica napus cv 212/86') and tobacco (Nicotiana tabacum cv Xanthi NC') were co-cultivated with a single Agrobacterium tumefaciens strain containing two binary plasmids. Genes from both plasmids were expressed in approximately 50% of the primary transformants. Progeny expressing only one of the transgenes were observed in about 50% of the co-transformed lines, indicating that the genes were inserted at different loci. This single-strain co-transformation method allowed the use of a selectable marker during plant regeneration and subsequent recovery of marker-free progeny.
共转化作为一种方法进行了研究，该方法允许在植物再生过程中使用选择标记，随后获得含有所需基因但缺乏标记基因的后代。将油菜（甘蓝型油菜品种212/86'）和烟草（烟草品种Xanthi NC'）与含有两个双元质粒的单一根癌农杆菌菌株共培养。来自两个质粒的基因在大约50%的初级转化体中表达。在大约50%的共转化株系中观察到仅表达其中一个转基因的后代，这表明这些基因插入到了不同的位点。这种单菌株共转化方法允许在植物再生过程中使用选择标记，并随后获得无标记的后代。
