Zhou Liya, Yuan Quan, Ai Xuhong, Chen Jianping, Lu Yuwen, Yan Fei
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
College of Plant Protection, Northwest A & F University, Yangling 712100, China.
Biology (Basel). 2022 Feb 19;11(2):332. doi: 10.3390/biology11020332.
Rice stripe virus (RSV) causes one of the most serious viral diseases of rice. RNA interference is one of the most efficient ways to control viral disease. In this study, we constructed an amiRNA targeting the RSV MP gene (amiR MP) based on the backbone sequence of the osa-MIR528 precursor, and obtained marker-free transgenic rice plants constitutively expressing amiR MP by s-mediated transformation. A transient expression assay demonstrated that dimeric amiR MP could be effectively recognized and cleaved at the target MP gene in plants. Northern blot of miRNA indicated that amiR MP-mediated viral resistance could be stably inherited. The transgenic rice plants were highly resistant to RSV (73-90%). Our research provides novel rice germplasm for RSV control.
水稻条纹病毒（RSV）引发水稻最严重的病毒病之一。RNA干扰是控制病毒病最有效的方法之一。在本研究中，我们基于osa-MIR528前体的骨干序列构建了靶向RSV MP基因的amiRNA（amiR MP），并通过农杆菌介导的转化获得了组成型表达amiR MP的无标记转基因水稻植株。瞬时表达分析表明，二聚体amiR MP在植物中能被有效识别并在目标MP基因处被切割。miRNA的Northern印迹表明，amiR MP介导的病毒抗性能够稳定遗传。转基因水稻植株对RSV具有高度抗性（73%-90%）。我们的研究为控制RSV提供了新的水稻种质。
