Genomics-assisted Breeding and Crop Improvement Laboratory, National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502324, Telangana, India.
Plant Physiol. 2019 May;180(1):253-275. doi: 10.1104/pp.18.00934. Epub 2019 Feb 8.
The identification of functionally relevant molecular tags is vital for genomics-assisted crop improvement and enhancement of seed yield, quality, and productivity in chickpea (). The simultaneous improvement of yield/productivity as well as quality traits often requires pyramiding of multiple genes, which remains a major hurdle given various associated epistatic and pleotropic effects. Unfortunately, no single gene that can improve yield/productivity along with quality and other desirable agromorphological traits is known, hampering the genetic enhancement of chickpea. Using a combinatorial genomics-assisted breeding and functional genomics strategy, this study identified natural alleles and haplotypes of an -type transporter gene that regulates seed weight, an important domestication trait, by transcriptional regulation and modulation of the transport of glutathione conjugates in seeds of and chickpea. The superior allele/haplotype of this gene introgressed in and near-isogenic lines enhances the seed weight, yield, productivity, and multiple desirable plant architecture and seed-quality traits without compromising agronomic performance. These salient findings can expedite crop improvement endeavors and the development of nutritionally enriched high-yielding cultivars in chickpea.
鉴定功能相关的分子标记对于基于基因组学的作物改良以及提高鹰嘴豆的种子产量、品质和生产力至关重要。()要同时提高产量/生产力以及品质性状，通常需要聚合多个基因，这是一个主要的障碍，因为存在各种相关的上位性和多效性效应。不幸的是，目前还没有一个单一的基因可以同时提高产量/生产力以及品质和其他理想的农艺形态性状，这阻碍了鹰嘴豆的遗传改良。本研究采用组合基因组辅助育种和功能基因组学策略，鉴定了一种 - 型转运蛋白基因的自然等位基因和单倍型，该基因通过转录调控和调节谷胱甘肽轭合物在 和 鹰嘴豆种子中的运输，调节种子重量这一重要的驯化性状。该基因在 和 近等基因系中的优异等位基因/单倍型的导入增强了种子重量、产量、生产力以及多个理想的植物结构和种子品质性状，而不会影响农艺性能。这些显著的发现可以加速作物改良工作以及开发营养丰富的高产鹰嘴豆品种。
