National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100, Teramo, Italy.
Dipartimento di Medicina Veterinaria, strada provinciale per Casamassima Km 3, 70100, Valenzano, Bari, Italy.
Sci Rep. 2019 Feb 8;9(1):1714. doi: 10.1038/s41598-018-37497-4.
Prompt identification of the causative pathogen of an infectious disease is essential for the choice of treatment or preventive measures. In this perspective, nucleic acids purified from the brain tissue of a dog succumbed after severe neurological signs were processed with the MinION (Oxford Nanopore Technologies, Oxford UK) sequencing technology. Canine distemper virus (CDV) sequence reads were detected. Subsequently, a specific molecular test and immunohistochemistry were used to confirm the presence of CDV RNA and antigen, respectively, in tissues. This study supports the use of the NGS in veterinary clinical practice with potential advantages in terms of rapidity and broad-range of molecular diagnosis.
确定传染病的病原体对于选择治疗或预防措施至关重要。从这个角度来看，从一只患有严重神经症状的狗的脑组织中提取的核酸，经 MinION（Oxford Nanopore Technologies，英国牛津）测序技术处理后，检测到犬瘟热病毒（CDV）序列。随后，使用特定的分子检测和免疫组织化学法分别确认了组织中 CDV RNA 和抗原的存在。本研究支持在兽医临床实践中使用 NGS，其在快速性和广泛的分子诊断方面具有潜在优势。
