Department of Environmental Sciences and Engineering, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
Division of Environmental Engineering, Graduate School of Engineering, Kyoto University, Kyoto, 6158540, Japan.
Environ Sci Pollut Res Int. 2019 Apr;26(10):9955-9965. doi: 10.1007/s11356-019-04433-6. Epub 2019 Feb 9.
Three sequential extraction procedures (SEPs), modified Tessier, modified BCR, and CIEMAT, were compared for mercury fractionation in polluted soils. With satisfactory total mercury recovery, the modified Tessier and modified BCR SEPs were comparable with each other in terms of extraction efficiency in equivalent mercury fractions, whereas both SEPs were not as efficient as the CIEMAT SEP. However, the CIEMAT SEP might underestimate the oxidizable mercury fractions due to the humic and fulvic complexes instead of the organic matter of the other two SEPs. For mercury bioavailability identification, based on Pearson correlation analysis, all fractions in each SEP were significantly correlated with mercury uptake in Ipomoea aquatica, causing difficulty in comparison. Partial correlation analysis indicated that the mobile mercury fractions extracted by the first step in all three SEPs had a positive correlation with mercury uptake by plant, while mercury bound to organic matter extracted by both modified Tessier and modified BCR SEPs presented negative correlation with mercury uptake by plant which was in contrast to CIEMAT SEP. Meanwhile, clearly positive correlations between mercury fractions extracted by the former three steps of CIEMAT SEP and mercury uptake in Ipomoea aquatica were observed, demonstrating that CIEMAT SEP provided more accurate results related to Hg bioavailability than did the other two SEPs.
三种连续提取程序（SEPs），即改良 Tessier、改良 BCR 和 CIEMAT，被用于比较污染土壤中汞的形态分析。在满意的总汞回收率下，改良 Tessier 和改良 BCR SEPs 在等效汞形态的提取效率方面彼此相当，而这两种 SEPs 均不如 CIEMAT SEP 有效。然而，由于 humic 和 fulvic 复合物而不是其他两种 SEPs 的有机物，CIEMAT SEP 可能会低估可氧化汞形态。对于汞生物有效性的识别，基于 Pearson 相关分析，每个 SEP 中的所有形态都与 Ipomoea aquatica 中的汞吸收显著相关，这使得比较变得困难。偏相关分析表明，所有三种 SEP 中第一步提取的可移动汞形态与植物吸收汞呈正相关，而改良 Tessier 和改良 BCR SEPs 提取的与有机物结合的汞与植物吸收汞呈负相关，这与 CIEMAT SEP 相反。同时，在 CIEMAT SEP 的前三个步骤中提取的汞形态与 Ipomoea aquatica 中的汞吸收之间观察到明显的正相关，表明与其他两种 SEPs 相比，CIEMAT SEP 提供了更准确的 Hg 生物有效性相关结果。
