State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovation Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovation Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Biol Macromol. 2019 May 15;129:339-350. doi: 10.1016/j.ijbiomac.2019.01.200. Epub 2019 Feb 6.
Sinorhodeus microlepis (S. microlepis) is recently described as a new species and represents a new genus Sinorhodeu of the subfamily Acheilognathinae. In this study, we first sequenced the complete mitogenome of S. microlepis and compared with the other 29 bitterling mitogenomes. The S. microlepis mitogenome is 16,591 bp in length and contains 37 genes. Gene distribution pattern is identical among 30 bitterling mitogenomes. A significant linear correlation between A+T% and AT-skew were found among 29 bitterling mitogenomes, except S. microlepis shows unusual AT-skew with slightly negative in tRNAs and PCGs. Bitterling mitogenomes exhibit highly conserved usage bias of start codon, relative synonymous codons and amino acids, overlaps and non-coding intergenic spacers. Phylogenetic trees constructed by 13 PCGs strongly support the polyphyly of the genus Acheilognathus and the paraphyly of Rhodeus and Tanakia. Together with the unusual characters of S. microlepis mitogenomes and phylogenetic trees, S. microlepis should be a sister species to the genus Rhodeu that might diverge about 13.69 Ma (95% HPD: 12.96-14.48 Ma).
黄河鱲（S. microlepis）最近被描述为一个新物种，代表了鱲科副鱲亚科的一个新属 Sinorhodeu。在本研究中，我们首次对 S. microlepis 的完整线粒体基因组进行了测序，并与其他 29 种鱲的线粒体基因组进行了比较。S. microlepis 的线粒体基因组长 16591bp，包含 37 个基因。30 种鱲的线粒体基因组基因分布模式相同。在 29 种鱲的线粒体基因组中发现 A+T%和 AT-skew 之间存在显著的线性相关性，但 S. microlepis 的 tRNAs 和 PCGs 中的 AT-skew 呈异常的负偏态分布。鱲的线粒体基因组显示出高度保守的起始密码子、相对同义密码子和氨基酸的使用偏好性、重叠和非编码基因间间隔区。由 13 个 PCGs 构建的系统发育树强烈支持了属 Acheilognathus 的多系性和 Rhodeus 和 Tanakia 的并系性。结合 S. microlepis 线粒体基因组和系统发育树的异常特征，S. microlepis 可能是 Rhodeu 属的姐妹种，它们大约在 13.69 Ma（95%HPD：12.96-14.48 Ma）时分化。
