Yang Tianyan, Liu Yuping, Ning Zijun
Fishery College, Zhejiang Ocean University, Zhoushan 316022, China.
Animals (Basel). 2023 Jan 20;13(3):362. doi: 10.3390/ani13030362.
The family Ophichthidae has the largest number and the most various species (about 359 valid species) in the order Anguilliformes worldwide. Both morphological and molecular characteristics have been used to assess their taxonomic status. However, due to the ambiguous morphological features, molecular data such as mitochondrial DNA sequences have been implemented for the correct identification and classification of these fishes. In this study, the gene arrangement and structure characteristics of two Ophichthidae mitochondrial genomes were investigated for the first time. The total mitogenome lengths of and were 17,759 bp and 17,856 bp, respectively. Comparing with the ancestral mitochondrial gene order, the irregular gene rearrangement happened between and tRNA-Pro (P) genes with another similar control region emerging between tRNA-Thr (T) and genes, which could be explained by the tandem duplication and random loss (TDRL) model appropriately. ML phylogenetic tree demonstrated that the family Ophichthidae was monophyletic origin, but genus might be polyphyletic because of the confused cluster relationships among different species.
蛇鳗科是全球鳗鲡目鱼类中种类数量最多且最为多样的一科（约359个有效物种）。形态学和分子特征均已被用于评估它们的分类地位。然而，由于形态特征模糊不清，线粒体DNA序列等分子数据已被用于这些鱼类的准确鉴定和分类。在本研究中，首次对两个蛇鳗科线粒体基因组的基因排列和结构特征进行了研究。和的线粒体基因组全长分别为17,759 bp和17,856 bp。与祖先线粒体基因顺序相比，和tRNA-脯氨酸（P）基因之间发生了不规则的基因重排，并且在tRNA-苏氨酸（T）和基因之间出现了另一个类似的控制区，这可以用串联重复和随机丢失（TDRL）模型进行恰当解释。最大似然法系统发育树表明，蛇鳗科起源于单系，但属可能是多系的，因为不同物种之间的聚类关系混乱。
