College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China.
College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China.
Plant Physiol Biochem. 2019 Apr;137:42-52. doi: 10.1016/j.plaphy.2019.01.028. Epub 2019 Jan 28.
Root system is essential for plants to absorb water and nutrients. The root related traits are complex quantitative traits and regulated by genetic control. Here, we used two association mapping panels to perform a genome-wide association study (GWAS) on seven root related traits in Brassica napus at the seedling stage and obtained 27 SNP loci significantly associated with the phenotypes. We further conducted a genome-wide LD block analysis of the candidate peak regions and obtained 295 candidate genes with high association peaks across seven phenotypes in LD region. In addition, a protein interaction network using the candidate genes identified here was constructed, and 113 genes were associated. Seven genes, BnaA03g47330D, BnaC09g16810D, BnaA06g22840D, BnaA03g28390D, BnaA08g19920D, BnaA03g28930D and BnaA03g11440D were in a large cluster, and may play important roles in interacting with other related genes. Our data may provide resources for molecular breeding and functional analysis of root growth and development in rapeseed.
根系是植物吸收水分和养分的基础。根系相关性状是复杂的数量性状，受遗传控制。本研究利用两个关联作图群体，对油菜苗期的 7 个根系相关性状进行了全基因组关联分析（GWAS），共获得 27 个与表型显著相关的 SNP 位点。进一步对候选峰区域进行全基因组 LD 块分析，在 LD 区域获得了 295 个与 7 个表型具有高关联峰值的候选基因。此外，利用鉴定的候选基因构建了蛋白质互作网络，发现有 113 个基因与之关联。7 个基因（BnaA03g47330D、BnaC09g16810D、BnaA06g22840D、BnaA03g28390D、BnaA08g19920D、BnaA03g28930D 和 BnaA03g11440D）位于一个大的簇中，可能在与其他相关基因相互作用中发挥重要作用。本研究结果可为油菜根系生长发育的分子育种和功能分析提供资源。
