IBIOM-CNR, Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, National Research Council, Italy.
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, C/ Pedro Cerbuna 12, 50009, Zaragoza, Spain.
Plant Physiol Biochem. 2019 Apr;137:53-61. doi: 10.1016/j.plaphy.2019.02.001. Epub 2019 Feb 2.
RNA editing is a widespread epitranscriptomic mechanism by which primary RNAs are specifically modified through insertions/deletions or nucleotide substitutions. In plants, RNA editing occurs in organelles (plastids and mitochondria), involves the cytosine to uridine modification (rarely uridine to cytosine) within protein-coding and non-protein-coding regions of RNAs and affects organelle biogenesis, adaptation to environmental changes and signal transduction. High-throughput sequencing technologies have dramatically improved the detection of RNA editing sites at genomic scale. Consequently, different bioinformatics resources have been released to discovery and/or collect novel events. Here, we review and describe the state-of-the-art bioinformatics tools devoted to the characterization of RNA editing in plant organelles with the aim to improve our knowledge about this fascinating but yet under investigated process.
RNA 编辑是一种广泛存在的表转录组学机制，通过该机制，初级 RNA 可以通过插入/缺失或核苷酸取代进行特异性修饰。在植物中，RNA 编辑发生在细胞器（质体和线粒体）中，涉及到蛋白质编码和非蛋白质编码 RNA 区域内胞嘧啶到尿嘧啶的修饰（很少有尿嘧啶到胞嘧啶），并影响细胞器的生物发生、对环境变化的适应和信号转导。高通量测序技术极大地提高了在基因组范围内检测 RNA 编辑位点的能力。因此，已经发布了不同的生物信息学资源来发现和/或收集新的事件。在这里，我们回顾和描述了用于描述植物细胞器中 RNA 编辑的最新生物信息学工具，旨在提高我们对这一迷人但研究不足的过程的认识。
