Ramadan Ahmed M, Al-Ghamdi Khalid M, Alghamdi Abdullah J, Amer Marwa, Ibrahim Mona I M, Atef Ahmed
Biological Science Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Princess Najla bint Saud Al-Saud Center for Excellence Research in Biotechnology, King Abdulaziz University, Jeddah, Saudi Arabia.
Saudi J Biol Sci. 2023 Nov;30(11):103817. doi: 10.1016/j.sjbs.2023.103817. Epub 2023 Sep 28.
Numerous studies have shown that stress in plant cells and organelles with transport electron chains is related to RNA editing. The ATP synthase complex present in mitochondria plays a crucial role in cellular respiration and consists of several subunits. Among them is the b subunit, which is encoded by the mitochondrial gene. Computing-based analysis of the effects of RNA editing of the gene in mitochondria leading to alterations in the b subunit of ATP synthase. Using the CLC Genomic Workbench 3, RNA editing analysis between the control and salt stress conditions was not significantly different. Depending on RNA editing, the tertiary structure model revealed a change in the states of the b subunit, reflecting differences in the central stalk and F1-catalytic domain. The study found that polar edits in the N-terminus of the b subunit allow for efficient H + ion selectivity and introduce a new coiled-coil alpha-helical structure that may help stabilize the complex. The most noteworthy finding of this study was the strong impact of these editing events on the tertiary structure of the b subunit, which has the potential to affect the ATPase activity and indicate that the editing in this subunit aimed to restore the original active protein and not as a response to salt stress.
大量研究表明，植物细胞和具有运输电子链的细胞器中的应激与RNA编辑有关。线粒体中存在的ATP合酶复合体在细胞呼吸中起关键作用，由几个亚基组成。其中b亚基由线粒体基因编码。基于计算分析线粒体中该基因的RNA编辑对ATP合酶b亚基改变的影响。使用CLC基因组工作台3，对照和盐胁迫条件之间的RNA编辑分析没有显著差异。根据RNA编辑，三级结构模型显示b亚基状态发生变化，反映了中心轴和F1催化结构域的差异。研究发现，b亚基N端的极性编辑允许有效的H +离子选择性，并引入一种新的卷曲螺旋α-螺旋结构，这可能有助于稳定复合体。这项研究最值得注意的发现是这些编辑事件对b亚基三级结构的强烈影响，这有可能影响ATP酶活性，并表明该亚基中的编辑旨在恢复原始活性蛋白，而不是作为对盐胁迫的反应。
