LE Ying, Zhang Xu-Pai, Xiong Yan-Qiu, Li Hong-Ying, Zhao Wei-Hua, Long Yuan, Luo Jun, Cheng Peng, Liu Zhen-Fang
Department of Hematology, The First Affliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
Department of Hematology, The First People's Hospital of Neijiang, Neijiang 641000, Sichuan Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2019 Feb;27(1):134-140. doi: 10.7534/j.issn.1009-2137.2019.01.022.
To detect the expression of miR-99a-5p in myelodysplastic syndrome (MDS), to predict the target genes and to analyze its function by using bioinformatics.
The expression levels of bone marrow miR-99a-5p in MDS patients were detected by qRT-PCR, and the correlation of miR-99a-5p expression with clinical pathological characteristics, percentage of marrow blasts , chromosome karyotype and peripheral blood hemogram were analyzed. The target genes of miR-99a-5p were predicted by Targetscan, Miranda and Microcosm, and the intersection of the predicted results of 3 softwares was used as a potential target gene for miR-99a-5p.
The expression level of bone marrow miR-99a-5p in MDS patients was significantly higher than that of healthy controls (P<0.01); According to the prognostic score of IPSS, MDS patients were divided into relatively low risk group (including low risk group and intermediate risk group 1) and relatively high risk group (including intermediate risk group 2 and high risk group). Analysis showed that the expression level of bone marrow miR-99a-5p in relatively low risk group was 2.40 times higher than that in healthy control group (P<0.01), the expression level of bone marrow miR-99a-5p in relatively high risk group was 6.66 times higher than that in healthy control group (P<0.01), the expression level of miR-99a-5p in relatively high risk group was 2.80 times higher than that in the relatively low risk group ( P<0.01) ; the expression level of bone marrow miR-99a-5p in t-MDS group was 6.35 times higher than that in healthy control group (P<0.001). There was a significant positive correlation between the expression level of miR-99a-5p and the percentage of marrow blasts (P<0.01), but the expression of miR-99a-5p did not correlate with chromosome karyotype and peripheral blood hemogram; In this study it was obtained that ST5 might participate in pathological mechanism of MDS by bioinformatic analyses and references.
The expression levels of miR-99a-5p is up-regulated in MDS patients, and its expression showed a rising tendency which may be involved in the pathogenesis of MDS by regulating target gene ST5.
检测骨髓增生异常综合征（MDS）中miR-99a-5p的表达，预测其靶基因并通过生物信息学分析其功能。
采用qRT-PCR检测MDS患者骨髓中miR-99a-5p的表达水平，并分析miR-99a-5p表达与临床病理特征、骨髓原始细胞百分比、染色体核型及外周血常规的相关性。通过Targetscan、Miranda和Microcosm预测miR-99a-5p的靶基因，将3种软件预测结果的交集作为miR-99a-5p的潜在靶基因。
MDS患者骨髓miR-99a-5p表达水平显著高于健康对照组（P<0.01）；根据IPSS预后评分，将MDS患者分为相对低危组（包括低危组和中危1组）和相对高危组（包括中危2组和高危组）。分析显示，相对低危组骨髓miR-99a-5p表达水平比健康对照组高2.40倍（P<0.01），相对高危组骨髓miR-99a-5p表达水平比健康对照组高6.66倍（P<0.01），相对高危组miR-99a-5p表达水平比相对低危组高2.80倍（P<0.01）；治疗相关MDS（t-MDS）组骨髓miR-99a-5p表达水平比健康对照组高6.35倍（P<0.001）。miR-99a-5p表达水平与骨髓原始细胞百分比呈显著正相关（P<0.01），但miR-99a-5p表达与染色体核型及外周血常规无关；通过生物信息学分析及参考文献，本研究得出ST5可能参与MDS的病理机制。
MDS患者中miR-99a-5p表达水平上调，其表达呈上升趋势，可能通过调节靶基因ST5参与MDS的发病机制。
