Laboratorio de Biología Molecular de Hongos y Plantas, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica AC, San Luis Potosí, SLP, Mexico.
Unidad de Biotecnología Vegetal, Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, Ags, Mexico.
Biochem Biophys Res Commun. 2019 Mar 19;510(4):545-550. doi: 10.1016/j.bbrc.2019.01.095. Epub 2019 Feb 6.
Plants have developed mechanisms that allow them to tolerate different abiotic stresses. Among these mechanisms, the accumulation of specific proteins such as dehydrins (DHNs) and aquaporins (AQPs) can protect other proteins from damage during dehydration and may allow the control of water loss, respectively. Although both types of proteins are involved in plant protection against dehydration stress, a direct interaction between them has not been explored. A previous screen to identify potential OpsDHN1 protein interactions revealed an aquaporin as a possible candidate. Here, we used the Bimolecular Fluorescence Complementation (BiFC) approach to investigate the direct interaction of the cactus OpsDHN1 protein with the Arabidopsis plasma membrane PIP family aquaporin AtPIP2B (At2G37170). Since AtPIP2B is a membrane protein and OpsDHN1 is a cytosolic protein that may be peripherally associated with membranes, we propose that OpsDHN1/AtPIP2B interaction takes place at cellular membranes. Furthermore, we also demonstrate the interaction of AtPIP2B with the three Arabidopsis dehydrins COR47 (AT1G20440), ERD10 (At1g20450), and RAB18 (At5g66400).
植物已经发展出多种机制来耐受不同的非生物胁迫。在这些机制中，特定蛋白质（如脱水素（DHN）和水通道蛋白（AQP））的积累可以保护其他蛋白质在脱水时免受损伤，并分别允许控制水分流失。尽管这两种类型的蛋白质都参与了植物对脱水胁迫的保护，但它们之间的直接相互作用尚未被探索。先前的筛选以鉴定潜在的 OpsDHN1 蛋白相互作用的研究揭示了一种水通道蛋白可能是候选蛋白。在这里，我们使用双分子荧光互补（BiFC）方法来研究仙人掌 OpsDHN1 蛋白与拟南芥质膜 PIP 家族水通道蛋白 AtPIP2B（At2G37170）的直接相互作用。由于 AtPIP2B 是一种膜蛋白，而 OpsDHN1 是一种可能与膜周边相关的细胞质蛋白，我们推测 OpsDHN1/AtPIP2B 相互作用发生在细胞膜上。此外，我们还证明了 AtPIP2B 与三种拟南芥脱水素 COR47（AT1G20440）、ERD10（At1g20450）和 RAB18（At5g66400）的相互作用。
