Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden.
Stockholm CF-Center, Department of Pediatrics, Karolinska University Hospital, Huddinge, Stockholm, Sweden.
Eur J Clin Microbiol Infect Dis. 2019 Jun;38(6):1071-1077. doi: 10.1007/s10096-019-03493-3. Epub 2019 Feb 9.
Cystic fibrosis (CF) patients become colonized by pathogenic bacteria as well as by Candida species. The interplay between different microorganisms may play a key role in the prognosis of CF. The aim of the study was to analyze the coexistence patterns of bacteria and Candida spp. in sputum samples of patients with CF and to compare these patterns with the results of patients with other respiratory disorders (ORD). Sputum samples from 130 patients with CF and 186 patients with ORD were cultured on six different agar plates promoting the growth of bacteria and yeasts. Bacterial and Candida species were identified with MALDI-TOF MS. Pathogenic bacteria were found in 69.2% of the sputum samples of the CF patients, and in 44.1% the patients with ORD. CF patients tended to have growth of Pseudomonas aeruginosa and Staphylococcus aureus in sputum more often than patients with ORD. Overall, there was no difference in the coexistence of pathogenic bacteria and Candida spp. in these patient groups. However, when analyzed at the species level, P. aeruginosa and S. aureus coexisted with Candida spp. more frequently in sputum samples of CF patients compared with patients with ORD. Also, when analyzed according to age, it was shown that the adult (≥ 18 years) CF patients had a higher rate of coexistence of any pathogenic bacteria and Candida spp. than the children with CF and the adult patients with ORD. The rate for colonization with Candida together with pathogenic bacteria is increased in adult patients with CF.
囊性纤维化 (CF) 患者会被致病菌和念珠菌定植。不同微生物之间的相互作用可能在 CF 的预后中发挥关键作用。本研究旨在分析 CF 患者痰液样本中细菌和念珠菌属的共存模式，并将这些模式与其他呼吸系统疾病 (ORD) 患者的结果进行比较。对 130 例 CF 患者和 186 例 ORD 患者的痰液样本进行培养，分别接种在促进细菌和酵母菌生长的六种不同琼脂平板上。采用 MALDI-TOF MS 鉴定细菌和念珠菌属的种属。在 69.2%的 CF 患者和 44.1%的 ORD 患者的痰液样本中发现了致病菌。CF 患者痰液中铜绿假单胞菌和金黄色葡萄球菌的生长倾向高于 ORD 患者。总体而言，这些患者群体中致病菌和念珠菌属的共存没有差异。然而，在种属水平分析时，CF 患者的痰液样本中，铜绿假单胞菌和金黄色葡萄球菌与念珠菌属共存的情况比 ORD 患者更为常见。此外，按年龄分析表明，成年 (≥18 岁) CF 患者与 CF 儿童和 ORD 成年患者相比，任何致病菌和念珠菌属共存的比例更高。CF 成年患者中同时定植念珠菌和致病菌的比例增加。
