Zhao Shanshan, Zhao Yan, Rogers Karyne M, Chen Ailiang, Zhang Tian, Yang Shuming
Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Institute of Quality Standard & Testing Technology for Agro-Products, Beijing, 100081, China.
Key Laboratory of Agro-product Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing, 100081, China.
Rapid Commun Mass Spectrom. 2019 Apr 30;33(8):803-810. doi: 10.1002/rcm.8411.
Isotope reference materials are essential to enable reliable and comparable isotope data across multiple laboratories. Although many reference materials already exist, the best reference materials should mimic the unknown samples, so new reference materials continue to evolve with the development of isotope research in new product areas.
Two defatted beef reference materials, CAAS-1801 and CAAS-1802, with substantially different δ C values (due to difference in dietary intake), have been prepared as reference materials for stable C and N isotope analysis of meat tissue. Homogeneity, and short- and long-term stability tests of these reference materials have been performed. The δ C and δ N values of both materials were measured for two-point isotopic normalization against international reference materials by elemental analyzer/isotope ratio mass spectrometry (EA/IRMS). A total of nine international laboratories were selected for the joint evaluation. Cochran statistical analysis yielded the values reported here.
The defatted beef reference material CAAS-1801 from Heilongjiang province has a δ C value of -13.58 ± 0.56‰ relative to VPDB and a δ N value of 4.23 ± 0.56‰ relative to N in air. The defatted beef reference material CAAS-1802 from Sichuan province has a δ C value of -25.03 ± 0.45‰ and a δ N value of 4.36 ± 0.69‰.
The two defatted beef reference materials were found to be isotopically stable across a range of ambient temperatures, and to have low volatility and toxicity, which enables them to be useful as stable isotope reference materials in the field of authentication and traceability of meat.
同位素参考物质对于在多个实验室获得可靠且可比的同位素数据至关重要。尽管已经存在许多参考物质，但最佳的参考物质应模仿未知样品，因此随着新产品领域同位素研究的发展，新的参考物质也在不断演变。
已制备了两种脱脂牛肉参考物质CAAS - 1801和CAAS - 1802，它们的δC值有显著差异（由于饮食摄入不同），用作肉类组织稳定碳和氮同位素分析的参考物质。对这些参考物质进行了均匀性以及短期和长期稳定性测试。通过元素分析仪/同位素比率质谱仪（EA/IRMS）针对国际参考物质对两种物质的δC和δN值进行了两点同位素归一化测量。总共选择了九个国际实验室进行联合评估。 Cochr an统计分析得出了此处报告的值。
来自黑龙江省的脱脂牛肉参考物质CAAS - 1801相对于VPDB的δC值为 - 13.58 ± 0.56‰，相对于空气中氮的δN值为4.23 ± 0.56‰。来自四川省的脱脂牛肉参考物质CAAS - 1802的δC值为 - 25.03 ± 0.45‰，δN值为4.36 ± 0.69‰。
发现这两种脱脂牛肉参考物质在一系列环境温度下同位素稳定，且挥发性和毒性低，这使得它们可作为肉类鉴定和溯源领域的稳定同位素参考物质。
