Cimini Donatella, Zaccariello Lucio, D'Ambrosio Sergio, Lama Licia, Ruoppolo Giovanna, Pepe Olimpia, Faraco Vincenza, Schiraldi Chiara
Department of Experimental Medicine, University of Campania L. Vanvitelli, Via de Crecchio 7, 80138 Naples, Italy.
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania L. Vanvitelli, Via Vivaldi 43, 81100 Caserta, Italy.
Biotechnol Biofuels. 2019 Feb 4;12:22. doi: 10.1186/s13068-019-1362-6. eCollection 2019.
Due to its wide range of applications in the food, pharmaceutical and chemical fields, microbial synthesis of succinic acid is receiving growing attention, generating already relevant industrial results, as well as fueling constant research for improvements. In order to develop a sustainable process, a special focus is now set on the exploitation and conversion of lignocellulosic biomasses into platform chemicals.
In the present work we used BPP7 in separated hydrolysis and fermentation experiments with as starting material. Fed-batch strategies showed a maximal production of about 37 g/L of succinic acid after 43 h of growth and a productivity of 0.9 g/L h on the pilot scale. Global mass balance calculations demonstrated a hydrolysis and fermentation efficiency of about 75%. Moreover, the application of a material flow analysis showed the obtainment of 88.5 and 52 % of succinic acid, per kg of virgin biomass and on the total generated output, respectively.
The use of fed-batch strategies for the growth of on improved the titer and productivity of succinic acid on pre-pilot scale. Process evaluation through material flow analysis showed successful results and predicted a yield of succinic acid of about 30% in a fed-batch process that uses as only carbon source also in the feed. Preliminary considerations on the possibility to achieve an energetic valorization of the residual solid coming from the fermentation process were also carried out.
由于琥珀酸在食品、制药和化工领域的广泛应用，微生物合成琥珀酸受到越来越多的关注，已经取得了相关的工业成果，并推动了持续的改进研究。为了开发可持续的工艺，目前特别关注木质纤维素生物质的开发和转化为平台化学品。
在本工作中，我们使用BPP7进行分批水解和发酵实验，以[具体原料]为起始原料。补料分批策略显示，在中试规模下，生长43小时后琥珀酸的最大产量约为37 g/L，生产率为0.9 g/L·h。整体质量平衡计算表明水解和发酵效率约为75%。此外，物料流分析的应用表明，每千克原始生物质和总产出中分别获得了88.5%和52%的琥珀酸。
在[具体原料]上采用补料分批策略进行[微生物名称]的生长，提高了中试规模下琥珀酸的产量和生产率。通过物料流分析进行的工艺评估显示了成功的结果，并预测在补料分批工艺中，以[具体原料]作为唯一碳源进料时，琥珀酸的产率约为30%。还对发酵过程中产生的残余固体进行能源增值的可能性进行了初步考虑。
