Department of Experimental Medicine, Section of Biotechnology, Medical Hystology and Molecular Biology, University of Campania L. Vanvitelli, Naples, Italy.
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania L. Vanvitelli, Caserta, Italy.
Biotechnol Prog. 2021 Sep;37(5):e3184. doi: 10.1002/btpr.3184. Epub 2021 Jul 5.
Microscale fermentation systems are important high throughput tools in clone selection, and bioprocess set up and optimization, since they provide several parallel experiments in controlled conditions of pH, temperature, agitation, and gas flow rate. In this work we evaluated the performance of biotechnologically relevant strains with different respiratory requirements in the micro-Matrix microbioreactor. In particular Escherichia coli K4 requires well aerated fermentation conditions to improve its native production of chondroitin-like capsular polysaccharide, a biomedically attractive polymer. Results from batch and fed-batch experiments demonstrated high reproducibility with those obtained on 2 L reactors, although highlighting a pronounced volume loss for longer-term experiments. Basfia succiniciproducens and Actinobacillus succinogenes need CO addition for the production of succinic acid, a building block with several industrial applications. Different CO supply modes were tested for the two strains in 24 h batch experiments and results well compared with those obtained on lab-scale bioreactors. Overall, it was demonstrated that the micro-Matrix is a useful scale-down tool that is suitable for growing metabolically different strains in simple batch process, however, a series of issues should still be addressed in order to fully exploit its potential.
微尺度发酵系统是克隆选择和生物工艺设置和优化的重要高通量工具，因为它们在 pH 值、温度、搅拌和气体流速的受控条件下提供了多个平行实验。在这项工作中，我们评估了具有不同呼吸需求的生物技术相关菌株在微矩阵微生物反应器中的性能。特别是大肠杆菌 K4 需要良好的通气发酵条件来提高其天然产生的类似软骨素的荚膜多糖的产量，这是一种具有吸引力的生物医学聚合物。分批和补料分批实验的结果与在 2L 反应器上获得的结果高度重现，尽管对于长期实验来说，体积损失明显。巴斯夫氏菌 succiniciproducens 和 Actinobacillus succinogenes 需要添加 CO 才能生产丁二酸，这是一种具有多种工业应用的建筑块。在 24 小时分批实验中，对两种菌株进行了不同的 CO 供应模式测试，并与实验室规模生物反应器上获得的结果进行了很好的比较。总的来说，结果表明微矩阵是一种有用的缩小规模工具，适用于在简单的分批过程中生长代谢不同的菌株，但是，为了充分发挥其潜力，仍然需要解决一系列问题。
