Wu Yaoying, Norberg Pamela K, Reap Elizabeth A, Congdon Kendra L, Fries Chelsea N, Kelly Sean H, Sampson John H, Conticello Vincent P, Collier Joel H
Department of Biomedical Engineering, Duke University, Durham, North Carolina, 27708, United States.
Department of Neurosurgery, Duke University Medical Center, Durham, North Carolina 27710, United States.
ACS Biomater Sci Eng. 2017 Dec 11;3(12):3128-3132. doi: 10.1021/acsbiomaterials.7b00561. Epub 2017 Sep 11.
A supramolecular peptide vaccine system was designed in which epitope-bearing peptides self-assemble into elongated nanofibers composed almost entirely of alpha-helical structure. The nanofibers were readily internalized by antigen presenting cells and produced robust antibody, CD4+ T-cell, and CD8+ T-cell responses without supplemental adjuvants in mice. Epitopes studied included a cancer B-cell epitope from the epidermal growth factor receptor class III variant (EGFRvIII), the universal CD4+ T-cell epitope PADRE, and the model CD8+ T-cell epitope SIINFEKL, each of which could be incorporated into supramolecular multi-epitope nanofibers in a modular fashion.
设计了一种超分子肽疫苗系统，其中携带表位的肽自组装成几乎完全由α-螺旋结构组成的细长纳米纤维。在小鼠中，这些纳米纤维很容易被抗原呈递细胞内化，并且在没有补充佐剂的情况下产生强烈的抗体、CD4 + T细胞和CD8 + T细胞反应。研究的表位包括来自表皮生长因子受体III类变体（EGFRvIII）的癌症B细胞表位、通用CD4 + T细胞表位PADRE和模型CD8 + T细胞表位SIINFEKL，每一种都可以以模块化方式整合到超分子多表位纳米纤维中。
