Ma Liuqing, Ma Pengfei, Li Dezhu
Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Plant Divers. 2018 Jul 18;40(5):232-237. doi: 10.1016/j.pld.2018.07.004. eCollection 2018 Oct.
Burmanniaceae is one major group within the monocot order Dioscoreales that has not had its plastome sequenced. Members of Burmanniaceae are mostly achlorophyllous, although the genus also includes autotrophs. Here, we report sequencing and analysis of the first Burmanniaceae plastid genome from L.. This plastome is 157,480 bp and was assembled as a circular sequence with the typical quadripartite structure of plant plastid genomes. This plastome has a regular number of potentially functional genes with a total of 111, including 78 protein coding genes, 4 ribosomal RNA (rRNA) genes, and 29 tRNA genes. The ratio of the total length of genic:intergenic DNA is 1.58:1, and the mean length of intergenic regions is 398 bp, the longest being 1918 bp. The overall GC content of the . plastome is 34.90%, and the IR regions in . are more GC rich (39.50%) than the LSC (32.30%) and SSC (28.80%) regions. Phylogenetic analysis of protein-coding sequences from plastomes of related species in the order Dioscoreales support a clade comprising Burmanniaceae and Dioscoreaceae. This phylogenetic placement is congruent with previous findings based on nuclear and mitochondrial evidence.
水玉簪科是单子叶植物薯蓣目内一个尚未对其质体基因组进行测序的主要类群。水玉簪科的成员大多无叶绿素，不过该属也包括自养植物。在此，我们报告了首个来自L.的水玉簪科质体基因组的测序及分析结果。这个质体基因组大小为157,480 bp，组装成了具有植物质体基因组典型四分体结构的环状序列。该质体基因组拥有正常数量的潜在功能基因，共计111个，包括78个蛋白质编码基因、4个核糖体RNA（rRNA）基因和29个转运RNA（tRNA）基因。基因DNA与基因间DNA的总长度之比为1.58:1，基因间区域的平均长度为398 bp，最长的为1918 bp。L.的质体基因组的总体GC含量为34.90%，L.的IR区域比LSC（32.30%）和SSC（28.80%）区域富含更多GC（39.50%）。对薯蓣目相关物种质体基因组中蛋白质编码序列的系统发育分析支持一个包含水玉簪科和薯蓣科的分支。这种系统发育位置与基于核和线粒体证据的先前发现一致。
