College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Biomed Res Int. 2021 Dec 1;2021:9410496. doi: 10.1155/2021/9410496. eCollection 2021.
DC. possesses both edible and medicinal properties and is widely distributed throughout China. In this study, the complete cp genome of was sequenced and assembled. The total length of the complete s cp genome was 151,977 bp, including an LSC region of 84,553 bp, SSC region of 18,138 bp, and IR region of 24,643 bp. Sequence analyses revealed that the cp genome encoded 132 genes, including 87 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The GC content was 37.6%. One hundred mononucleotide microsatellites, 4 dinucleotide microsatellites, 67 trinucleotide microsatellites, 4 tetranucleotide microsatellites, and 1 long repeat were identified. The SSR frequency of the LSC region was significantly greater than that of the IR and SSC regions. In total, 175 SSRs and highly variable regions were recognized as potential cp markers. By analyzing the IR/LSC and IR/SSC boundaries, structural differences between . and 6 other species were detected. According to phylogenetic analyses, was most closely related to . and . Overall, this study provides complete cp genome resources for that will be beneficial for identifying potential molecular markers and evolutionary patterns of and its closely related species.
滇丁香（DC.）兼具食用和药用价值，在中国广泛分布。本研究对滇丁香的完整 cp 基因组进行了测序和组装。滇丁香的 cp 基因组全长为 151977bp，包括一个 84553bp 的 LSC 区、一个 18138bp 的 SSC 区和一个 24643bp 的 IR 区。序列分析表明，该 cp 基因组编码了 132 个基因，包括 87 个蛋白质编码基因、37 个 tRNA 基因和 8 个 rRNA 基因。GC 含量为 37.6%。鉴定出 100 个单核苷酸微卫星、4 个二核苷酸微卫星、67 个三核苷酸微卫星、4 个四核苷酸微卫星和 1 个长重复。LSC 区的 SSR 频率显著高于 IR 和 SSC 区。共识别出 175 个 SSR 和高度可变区，可作为潜在的 cp 标记。通过分析 IR/LSC 和 IR/SSC 边界，检测到滇丁香与其他 6 个物种之间的结构差异。根据系统发育分析，滇丁香与. 关系最为密切。总体而言，本研究为滇丁香提供了完整的 cp 基因组资源，有助于识别滇丁香及其近缘种的潜在分子标记和进化模式。
