College of Food Science and Pharmaceutical Science, Xinjiang Agricultural University, Urumqi, China.
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
J Liposome Res. 2020 Mar;30(1):37-44. doi: 10.1080/08982104.2019.1580720. Epub 2019 Mar 19.
The aim of this study was to evaluate whether lycopene-loaded liposomes (L-LYC) could interfere with the antitumor efficacy and cardiotoxicity of doxorubicin (DOX). L-LYC were prepared by a thin-film hydration method to overcome the instability, insolubility, and low bioavailability of lycopene. The mean diameter and morphology of the liposomes were determined by dynamic light scattering and transmission electron microscopy, respectively, and then, cytotoxicity and antitumor activity were determined to evaluate the effects of L-LYC and their combination with DOX. Finally, we evaluated whether L-LYC could decrease the DOX-induced cardiotoxicity . The results showed that the particle size of L-LYC appeared uniform, and the average diameter was approximately 160.4 nm. Compared with DOX treatment alone, the combination of L-LYC and DOX showed significantly increased cytotoxicity and decreased the tumor size in B16 melanoma-bearing mice . Furthermore, the DOX-induced cardiotoxicity was clearly relieved in combination with L-LYC. The overall findings indicated that L-LYC have a great potential for improving the therapeutic efficacy and attenuating the cardiotoxicity of the chemotherapy drug DOX.
本研究旨在评估番茄红素脂质体（L-LYC）是否能干扰阿霉素（DOX）的抗肿瘤疗效和心脏毒性。通过薄膜水化法制备 L-LYC，以克服番茄红素的不稳定性、不溶性和低生物利用度。通过动态光散射和透射电子显微镜分别测定脂质体的平均直径和形态，然后测定细胞毒性和抗肿瘤活性，以评估 L-LYC 及其与 DOX 联合应用的效果。最后，我们评估了 L-LYC 是否能降低 DOX 引起的心脏毒性。结果表明，L-LYC 的粒径均匀，平均直径约为 160.4nm。与单独 DOX 处理相比，L-LYC 和 DOX 的联合应用显示出明显增加的细胞毒性和降低 B16 黑素瘤荷瘤小鼠的肿瘤体积。此外，与 L-LYC 联合应用明显减轻了 DOX 引起的心脏毒性。总的来说，这些发现表明 L-LYC 具有提高化疗药物 DOX 的治疗效果和减轻心脏毒性的巨大潜力。
