National Institute for Laser, Plasma and Radiation Physics, Magurele, Ilfov, Romania.
National Institute for Laser, Plasma and Radiation Physics, Magurele, Ilfov, Romania; ELI-NP, "Horia Hulubei" National Institute for Physics and Nuclear Engineering, Magurele, Ilfov, Romania.
Microb Pathog. 2019 Apr;129:250-256. doi: 10.1016/j.micpath.2019.02.012. Epub 2019 Feb 8.
Aqueous chlorpromazine solutions exposed to 266 nm generated as fourth harmonic of Nd:YAG pulsed laser along time intervals from 1 min to 240 min were investigated for their antimicrobial activity against planktonic and adherent Gram-positive bacterial strains. Qualitative and quantitative assays based on microbiological methods and flow cytometry assays were performed to establish the minimum inhibitory and minimum biofilm eradication concentrations and to reveal some of the possible mechanisms of antimicrobial activity. Optimal irradiation conditions and combinations of photoproducts for achieving the best antimicrobial and antibiofilm effects are suggested. It was confirmed that chlorpromazine solutions irradiated for 15 min and 30 min have the best antimicrobial and antibiofilm activity against Staphylococcus aureus ATCC 6538, methicillin susceptible Staphylococcus aureus, methicillin resistant Staphylococcus aureus, Enterococcus faecium 17-VAR, Enterococcus faecalis 2921, and Bacillus subtilis 6633. Flow cytometry revealed that two of the possible mechanisms of the antimicrobial activity of irradiated chlorpromazine are the inhibition of efflux pumps activity and induction of cellular membrane lesions.
将暴露于 266nm 的水合氯丙嗪溶液作为 Nd:YAG 脉冲激光的第四谐波，在 1 分钟至 240 分钟的时间间隔内进行研究，以评估其对浮游和附着革兰氏阳性细菌菌株的抗菌活性。基于微生物学方法和流式细胞术检测进行定性和定量分析，以确定最小抑菌和最小生物膜清除浓度，并揭示一些可能的抗菌活性机制。建议了最佳的辐照条件和光产物组合，以实现最佳的抗菌和抗生物膜效果。结果证实，辐照 15 分钟和 30 分钟的氯丙嗪溶液对金黄色葡萄球菌 ATCC 6538、甲氧西林敏感金黄色葡萄球菌、甲氧西林耐药金黄色葡萄球菌、屎肠球菌 17-VAR、粪肠球菌 2921 和枯草芽孢杆菌 6633 具有最佳的抗菌和抗生物膜活性。流式细胞术揭示了辐照氯丙嗪抗菌活性的两种可能机制是抑制外排泵活性和诱导细胞膜损伤。
