Udrea Ana Maria, Staicu Angela, Smarandache Adriana, Andrei Ionut Relu, Badea Madalina Andreea, Avram Speranta, Pascu Mihail Lucian, Pirvulescu Ruxandra Angela, Balas Mihaela
National Institute for Laser, Plasma and Radiation Physics (INFLPR), 409 Atomistilor Street, Magurele, 077125, Romania.
Research Institute of the University of Bucharest (ICUB), University of Bucharest, 90-92 Sos. Panduri, Bucharest, 050663, Romania.
Sci Rep. 2024 Dec 5;14(1):30329. doi: 10.1038/s41598-024-82088-1.
Breast cancer remains a global health challenge, prompting interest in the anticancer properties of other drugs, including chlorpromazine (CPZ). This study presents a novel approach in breast cancer treatment using laser irradiated CPZ. CPZ dissolved in distilled water, was exposed to 266 nm laser irradiation for varying durations, characterized by UV-Vis and FTIR spectroscopy, followed by drug-likeness and ADME-Tox predictions. In vitro assays evaluated the cytotoxicity and cellular effects on MCF-7 breast cancer cells, and compared with MCF-12 A healthy cell line. Laser irradiation altered CPZ molecular structure resulting in photoproducts with favourable drug-like properties and ADME-Tox profiles. In vitro evaluations demonstrate dose and irradiation time-dependent cytotoxicity against breast cancer cells, and reduced toxicity on healthy cell line. Significant alterations in F-actin organization, and excessive ROS generation were also proved, suggesting the potential of laser-modified CPZ for breast cancer therapy. This study introduces a novel approach to breast cancer treatment through laser irradiated CPZ, highlighting promising advancements in therapy and emphasizing the role of laser-generated compounds.
乳腺癌仍然是一项全球性的健康挑战，这引发了人们对包括氯丙嗪（CPZ）在内的其他药物抗癌特性的兴趣。本研究提出了一种使用激光照射CPZ治疗乳腺癌的新方法。将溶解于蒸馏水中的CPZ暴露于266 nm激光照射不同时长，通过紫外可见光谱和傅里叶变换红外光谱进行表征，随后进行类药性质和药物代谢及毒性预测。体外试验评估了对MCF-7乳腺癌细胞的细胞毒性和细胞效应，并与MCF-12 A健康细胞系进行比较。激光照射改变了CPZ的分子结构，产生了具有良好类药性质和药物代谢及毒性特征的光产物。体外评估表明，对乳腺癌细胞具有剂量和照射时间依赖性的细胞毒性，对健康细胞系的毒性降低。还证实了F-肌动蛋白组织的显著改变和过量活性氧的产生，表明激光改性CPZ在乳腺癌治疗中的潜力。本研究通过激光照射CPZ引入了一种治疗乳腺癌的新方法，突出了治疗方面有前景的进展，并强调了激光生成化合物的作用。
