Vélez Carolina, Clauzure Mariángeles, Williamson Delia, Koncurat Mirta A, Santa-Coloma Tomás A, Barbeito Claudio
Faculty of Veterinary Science, National University of La Pampa (UNLPam), Argentina; National Scientific and Technical Research Council (CONICET), Argentina.
National Scientific and Technical Research Council (CONICET), Argentina; Institute for Biomedical Research (BIOMED, CONICET-UCA), Laboratory of Cellular and Molecular Biology, School of Medical Sciences, Pontifical Catholic University of Argentina (UCA), Buenos Aires, Argentina.
Theriogenology. 2019 Apr 1;128:133-139. doi: 10.1016/j.theriogenology.2019.01.017. Epub 2019 Jan 25.
In pigs, given the type of epitheliochorial and non-invasive placenta, the trophoblast is in intimate contact with maternal tissues. The dialogue established between the conceptus and the endometrium involves, among others, the immune system, which minimizes the chances of rejection of the embryo and promotes the establishment of pregnancy. The aim of this work was to determine the concentration of IL-1β, IL-2 and IL-4 in sera and in extracts of maternal and fetal placenta from sows of different gestational periods. Reproductive tracts from 23 crossbreed sows, between 30 and 114 days of gestation (dg), and from 8 non-pregnant sows were used. The concentration of the cytokines was determined by ELISA. IL-1β, IL-2 and IL-4 demonstrated a similar pattern of concentration at the placental interface and serum; they were found elevated in tissues at 30 and 60-70 dg, and significantly decreased at term, period in which the cytokines were significantly increased in serum. These results show that IL-1β, IL-2, and IL-4 are differentially modulated during pregnancy and at term, and suggest an important role of these cytokines in defining the proinflammatory stage of these periods.
在猪中，鉴于其上皮绒毛膜型和非侵入性胎盘的类型，滋养层与母体组织紧密接触。胚胎与子宫内膜之间建立的对话涉及多个方面，其中包括免疫系统，该系统可将胚胎被排斥的几率降至最低，并促进妊娠的建立。这项工作的目的是测定不同妊娠期母猪血清以及母体和胎儿胎盘提取物中白细胞介素-1β（IL-1β）、白细胞介素-2（IL-2）和白细胞介素-4（IL-4）的浓度。使用了23头妊娠期在30至114天（dg）的杂交母猪以及8头未怀孕母猪的生殖道。通过酶联免疫吸附测定法（ELISA）测定细胞因子的浓度。IL-1β、IL-2和IL-4在胎盘界面和血清中的浓度呈现相似模式；在30天以及60 - 70天dg时，它们在组织中的浓度升高，而在足月时显著降低，不过在足月时血清中的细胞因子浓度显著增加。这些结果表明，IL-1β、IL-2和IL-4在妊娠期间和足月时受到不同程度的调节，并提示这些细胞因子在确定这些时期的促炎阶段中发挥重要作用。
