Jana Barbara, Andronowska Aneta, Całka Jarosław, Mówińska Aleksandra
Division of Reproductive Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, Olsztyn, 10-748, Poland.
Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, Olsztyn, 10-719, Poland.
Sci Rep. 2025 Jan 22;15(1):2806. doi: 10.1038/s41598-025-86787-1.
An inflammatory response is related to different inflammatory mediators generated by immune and endometrial cells. The links between lipopolysaccharide (LPS), cytokines, and leukotrienes (LTs) in endometrial stromal cells remain unclear. This study aimed to examine the influence of LPS, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-4 and IL-10 on 5-lipooxygenase (5-LO), LTA4 hydrolase (LTAH) and LTC4 synthase (LTCS) mRNA and protein abundances, and LTB4 and cysteinyl (cys)-LTs release including LTC4, by the cultured pig endometrial stromal cells, as well as on cell viability. 24-hour exposure to LPS, TNF-α, IL-4 and IL-10 up-regulated 5-LO mRNA and protein abundances. LPS increased LTAH mRNA abundance, while TNF-α, IL-1β and IL-10 augmented LTAH mRNA and protein abundances. TNF-α and IL-4 increased LTCS mRNA and protein abundances. In addition, LTCS mRNA abundance was enhanced by LPS and IL-4, while LTCS protein abundance was increased by IL-1β. Cells responded to LPS, TNF-α, IL-1β and IL-10 with increased LTB4 release. TNF-α, IL-1β and IL-4 stimulated LTC4 release. Cys-LTs release was up-regulated by LPS, TNF-α, IL-1β and IL-4. All studied cytokines augmented cell viability. In summary, LPS, TNF-α, IL-1β, IL-4 and IL-10 are potential LTs immunomodulatory agents in endometrial stromal cells. These functional interactions could be one of the mechanisms responsible for local orchestrating events in inflamed and healthy endometrium.
炎症反应与免疫细胞和子宫内膜细胞产生的不同炎症介质有关。子宫内膜基质细胞中脂多糖（LPS）、细胞因子和白三烯（LTs）之间的联系仍不清楚。本研究旨在检测LPS、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β、IL-4和IL-10对培养的猪子宫内膜基质细胞中5-脂氧合酶（5-LO）、LTA4水解酶（LTAH）和LTC4合酶（LTCS）mRNA和蛋白丰度以及LTB4和半胱氨酰（cys）-LTs释放（包括LTC4）的影响，以及对细胞活力的影响。24小时暴露于LPS、TNF-α、IL-4和IL-10可上调5-LO mRNA和蛋白丰度。LPS增加LTAH mRNA丰度，而TNF-α、IL-1β和IL-10增加LTAH mRNA和蛋白丰度。TNF-α和IL-4增加LTCS mRNA和蛋白丰度。此外，LPS和IL-4增强LTCS mRNA丰度，而IL-1β增加LTCS蛋白丰度。细胞对LPS、TNF-α、IL-1β和IL-10的反应是LTB4释放增加。TNF-α、IL-1β和IL-4刺激LTC4释放。LPS、TNF-α、IL-1β和IL-4上调cys-LTs释放。所有研究的细胞因子均增强细胞活力。总之，LPS、TNF-α、IL-1β、IL-4和IL-10是子宫内膜基质细胞中潜在的LTs免疫调节因子。这些功能相互作用可能是炎症和健康子宫内膜中局部协调事件的机制之一。
