State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi, Jiangsu, China; (Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou, Jiangsu, China.
J Nutr Biochem. 2019 Apr;66:43-51. doi: 10.1016/j.jnutbio.2019.01.007. Epub 2019 Jan 18.
Depression disorder is rapidly advancing worldwide, and therapeutic strategy through gut-brain axis has been proven to be effective in the treatment. Here we studied the effect of lactic acid bacteria (LAB) treatment on depression. C57BL/6J mice were administered with LAB during a 5-week chronic unpredictable mild stress. Bifidobacterium longum subsp. infantis E41 and Bifidobacterium breve M2CF22M7, which improved the expression of Tph1 and secretion of 5-hydroxytryptophan (5-HTP) in RIN14B cells, significantly reduced depressive behaviors of mice in the forced swim test, sucrose preference test and step-down test, as well as increased the level of 5-hydroxytryptamine and brain-derived neurotrophic factor concentration in brain. Besides, M2CF22M7 reduced the serum corticosterone level. E41 increased cecal butyrate level, which significantly and positively correlated with some depression-related indexes. Using 16S rRNA-amplicon sequencing of faces, E41 and M2CF22M7 were found to improve the chronic-stress-induced microbial dysbiosis. They also normalized the host's pathways involving metabolism and gene information processing. These results indicate that Bifidobacterium E41 and M2CF22M7 have an antidepressant effect in mice partly in a 5-HTP dependent and microbiota-regulating manner. Nurturing the gut microbiota with these strains may become an emerging therapeutic way for mood disorder.
抑郁障碍在全球范围内迅速蔓延，通过肠-脑轴的治疗策略已被证明在治疗中有效。在这里，我们研究了乳酸菌（LAB）治疗对抑郁的影响。C57BL/6J 小鼠在 5 周的慢性不可预测轻度应激中给予 LAB。改善 RIN14B 细胞中 Tph1 表达和 5-羟色氨酸（5-HTP）分泌的长双歧杆菌亚种婴儿 E41 和短双歧杆菌 M2CF22M7，显著减少了强迫游泳试验、蔗糖偏好试验和跳下试验中小鼠的抑郁行为，以及增加了脑中 5-羟色胺和脑源性神经营养因子的浓度。此外，M2CF22M7 降低了血清皮质酮水平。E41 增加了盲肠丁酸盐水平，其与一些与抑郁相关的指标呈显著正相关。通过面部 16S rRNA 扩增子测序，发现 E41 和 M2CF22M7 改善了慢性应激引起的微生物失调。它们还使宿主涉及代谢和基因信息处理的途径正常化。这些结果表明，双歧杆菌 E41 和 M2CF22M7 以 5-HTP 依赖和微生物群调节的方式在小鼠中具有抗抑郁作用。用这些菌株培养肠道微生物群可能成为治疗情绪障碍的一种新兴治疗方法。
