Lecoq Hervé, Dafalla Gasim, Delécolle Brigitte, Wipf-Scheibel Catherine, Desbiez Cécile
INRA, UR407, Station de Pathologie Végétale, B.P. 94, 84143 Montfavet Cedex, France.
Plant Pathology Center, University of the Gezira, Wad Medani, Sudan.
Plant Dis. 2011 Feb;95(2):153-157. doi: 10.1094/PDIS-06-10-0447.
A virus isolate (Su-95-67) was obtained from a snake melon (Cucumis melo var. flexuosus) plant presenting severe chlorotic spots, mosaic, stunting, and leaf deformations collected in Eastern Sudan in 1995. Su-95-67 was easily mechanically transmissible and had a host range limited to a few cucurbit species. Isometric virus particles approximately 30 nm in diameter were observed in leaf dip preparations. A cytopathological study did not reveal alterations specific for a virus genus or family. A polyclonal antiserum was obtained and used in double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Su-95-67 was transmitted by seed at a low rate, by the red melon beetle (Aulacophora foveicollis), but not by the melon aphid (Aphis gossypii). Because Su-95-67 shared several properties with sobemoviruses, generic Sobemovirus reverse-transcription polymerase chain reaction primers were developed. They allowed amplification of a 384-bp fragment from extracts of plants infected by two sobemoviruses or by Su-95-67 but not from healthy plants extracts. Sequence comparison confirmed that Su-95-67 belongs to a new tentative Sobemovirus species for which we propose the name Snake melon asteroid mosaic virus (SMAMV). DAS-ELISA tests conducted on extracts of virus-infected cucurbit plants collected from 1992 to 2003 revealed the presence of SMAMV in 10.2% of 600 samples originating from different regions of Sudan.
1995年，在苏丹东部采集到一株呈现严重褪绿斑点、花叶、植株矮化和叶片变形症状的蛇瓜（Cucumis melo var. flexuosus）植株，从中分离出一种病毒（Su-95-67）。Su-95-67易于机械传播，寄主范围局限于少数几种葫芦科植物。在叶片浸渍制备物中观察到直径约30 nm的等轴病毒粒子。细胞病理学研究未发现特定病毒属或病毒科的特征性变化。制备了多克隆抗血清，并用于双抗体夹心酶联免疫吸附测定（DAS-ELISA）。Su-95-67能以低比率通过种子传播，可由红瓜甲虫（Aulacophora foveicollis）传播，但不能由瓜蚜（Aphis gossypii）传播。由于Su-95-67与南方菜豆花叶病毒属病毒具有若干共同特性，因此开发了南方菜豆花叶病毒属通用的逆转录聚合酶链反应引物。这些引物能从感染两种南方菜豆花叶病毒属病毒或Su-95-67的植物提取物中扩增出一段384 bp的片段，但不能从健康植物提取物中扩增出该片段。序列比较证实，Su-95-67属于一种新的暂定南方菜豆花叶病毒属病毒种，我们将其命名为蛇瓜小行星花叶病毒（SMAMV）。对1992年至2003年采集的病毒感染葫芦科植物提取物进行的DAS-ELISA检测显示，在来自苏丹不同地区的600个样本中，有10.2%存在SMAMV。
