Banach Paulina, Dereziński Paweł, Matuszewska Eliza, Matysiak Jan, Bochyński Hubert, Kokot Zenon J, Nowak-Markwitz Ewa
Gynecologic Oncology Department, Poznan University of Medical Sciences, Polna 33, 60-535 Poznan, Poland.
Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznan, Poland.
Metabolites. 2019 Feb 9;9(2):30. doi: 10.3390/metabo9020030.
Gestational trophoblastic disease (GTD) is a group of highly aggressive, rare tumors. Human chorionic gonadotropin is a common biomarker used in the diagnosis and monitoring of GTD. To improve our knowledge of the pathology of GTD, we performed protein-peptide profiling on the urine of patients affected with gestational trophoblastic neoplasm (GTN). We analyzed urine samples from patients diagnosed with GTN ( = 26) and from healthy pregnant and non-pregnant controls ( = 17) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Ions were examined in a linear mode over a m/z range of 1000⁻10,000. All GTN urine samples were analyzed before and after treatment and compared with those of the controls. The statistical analyses included multivariate classification algorithms as well as ROC curves. Urine sample analyses revealed there were significant differences in the composition of the ions between the evaluated groups. Comparing the pre-treatment and group with the pregnant controls, we identified two discriminatory proteins: hemoglobin subunit α (m/z = 1951.81) and complement C4A (m/z = 1895.43). Then, comparing urine samples from the post-treatment cases with those from the non-pregnant controls, we identified the peptides uromodulin fragments (m/z = 1682.34 and 1913.54) and complement C4A (m/z = 1895.43).
妊娠滋养细胞疾病（GTD）是一组侵袭性很强的罕见肿瘤。人绒毛膜促性腺激素是用于GTD诊断和监测的常见生物标志物。为了增进我们对GTD病理学的了解，我们对妊娠滋养细胞肿瘤（GTN）患者的尿液进行了蛋白质-肽谱分析。我们使用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）分析了26例被诊断为GTN的患者以及17例健康孕妇和非孕妇对照的尿液样本。在1000⁻10,000的m/z范围内以线性模式检测离子。所有GTN尿液样本在治疗前后均进行了分析，并与对照组的样本进行比较。统计分析包括多变量分类算法以及ROC曲线。尿液样本分析显示，评估组之间离子组成存在显著差异。将治疗前的组与孕妇对照组进行比较，我们鉴定出两种具有鉴别意义的蛋白质：血红蛋白亚基α（m/z = 1951.81）和补体C4A（m/z = 1895.43）。然后，将治疗后病例的尿液样本与非孕妇对照组的样本进行比较，我们鉴定出肽段尿调节蛋白片段（m/z = 1682.34和1913.54）和补体C4A（m/z = 1895.43）。
