Watanabe A, Araki S, Kobari S, Sudo H, Tsuchida T, Uno T, Kosaka N, Shimomura K, Yamazaki M, Saito K
Faculty of Pharmaceutical Sciences, Research Center of Medicinal Resources, Laboratory of Molecular Biology and Biotechnology, Chiba University, Yayoi-cho 1-33, Inage-ku, Chiba 263-8522, Japan e-mail:
Basic Research Laboratory, Kanebo Co. Ltd., Tomobuchi-cho 1-5-90, Miyakojima-ku, Osaka 534-0016, Japan, , , , , , JP.
Plant Cell Rep. 1998 Dec;18(3-4):187-192. doi: 10.1007/s002990050554.
Angelica acutiloba, a medicinal plant used as a natural medicine Touki, was clonally propagated through axillary buds in vitro. No substantial differences were found in the random amplified polymorphic DNA (RAPD) pattern between the original A. acutiloba and the plant propagated in vitro, suggesting no changes in the DNA sequences and structure during in vitro propagation. The genetic similarities of several Angelica plants were investigated by restriction fragment length polymorphism (RFLP) and RAPD analyses. The RFLP and RAPD patterns of A. sinensis Diels were substantially different from those of A. acutiloba. Using ten different restriction enzymes, no RFLP was observed in the varieties of A. acutiloba. By RAPD analysis, A. acutiloba varieties can be classified into two major subgroups, i.e., A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyamae Hikino. The varieties of A. acutiloba Kitagawa in Japan and Angelica spp. in northeast China exhibited a very close genetic relationship.
当归（Angelica acutiloba），一种用作天然药材独活的药用植物，通过腋芽进行了离体克隆繁殖。在原始当归和离体繁殖的植株之间，随机扩增多态性DNA（RAPD）图谱未发现实质性差异，这表明在离体繁殖过程中DNA序列和结构没有变化。通过限制性片段长度多态性（RFLP）和RAPD分析研究了几种当归植物的遗传相似性。当归（A. sinensis Diels）的RFLP和RAPD图谱与当归（A. acutiloba）的图谱有很大不同。使用十种不同的限制性内切酶，在当归的变种中未观察到RFLP。通过RAPD分析，当归变种可分为两个主要亚组，即北川当归（A. acutiloba Kitagawa）和北川当归玉山变种（A. acutiloba Kitagawa var. sugiyamae Hikino）。日本的北川当归变种与中国东北的当归属植物表现出非常密切的遗传关系。
