Research Center for Medicinal Plant Resources, National Institutes of Biomedical Innovation, Health, and Nutrition, Ohashi 108-4, Nayoro, Hokkaido, 096-0065, Japan.
Research Center for Medicinal Plant Resources, National Institutes of Biomedical Innovation, Health, and Nutrition, Hachimandai 1-2, Tsukuba, Ibaraki, 305-0843, Japan.
J Nat Med. 2018 Jun;72(3):774-778. doi: 10.1007/s11418-018-1190-1. Epub 2018 Feb 20.
The root of Angelica acutiloba Kitagawa is an important crude drug in Kampo medicines (traditional Japanese medicine). Chemical evaluation of crude drugs is crucial to ensuring the safety and efficacy of herbal medicine; however, there is currently no chemical standard for the A. acutiloba crude drug in Japanese pharmacopoeia. (E)-ferulic acid (FA) is an important active ingredient of Angelica spp., including A. sinensis (Oliv.) Diels, and has been suggested as a marker for quality evaluation of those crude drugs. However, it has been controversial whether FA is a reliable marker constituent of A. acutiloba. To achieve effective extraction of FA from A. acutiloba, we compared three different extraction methods: alkaline hydrolysis, ethanol extraction, and hexane extraction. FA levels in these extracts were assessed using high performance liquid chromatography (HPLC), and alkaline hydrolysis was found to be the most effective. Furthermore, in the hydrolysate, FA was distinctly identified by thin layer chromatography (TLC) analysis. These results provide useful information for the quality control of the A. acutiloba crude drug.
当归属植物当归的根是日本汉方药（传统的日本药）中的一种重要的生药。生药的化学评价对于确保草药的安全性和有效性至关重要；然而，目前日本药典中还没有当归生药的化学标准。（E）-阿魏酸（FA）是当归属植物（包括当归 A. sinensis （Oliv.） Diels）的一种重要活性成分，已被提议作为这些生药质量评价的标志物。然而，FA 是否是当归的可靠标志物成分一直存在争议。为了从当归中有效提取 FA，我们比较了三种不同的提取方法：碱水解、乙醇提取和正己烷提取。使用高效液相色谱法（HPLC）评估这些提取物中的 FA 水平，发现碱水解法最为有效。此外，通过薄层色谱（TLC）分析在水解产物中明显鉴定出 FA。这些结果为当归生药的质量控制提供了有用的信息。
