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质体基因组中atpF和atpA基因之间间隔区的序列能够区分三种药用当归变种。

The sequences of the spacer region between the atpF and atpA genes in the plastid genome allows discrimination among three varieties of medicinal Angelica.

作者信息

Hosokawa Keizo, Hishida Atsuyuki, Nakamura Ikuo, Shibata Toshiro

机构信息

Department of Nutritional Management, Faculty of Health Sciences, Hyogo University, Kakogawa, Hyogo, Japan.

出版信息

Planta Med. 2006 May;72(6):570-1. doi: 10.1055/s-2005-916257.

DOI:10.1055/s-2005-916257
PMID:16773544
Abstract

The dried roots of Angelica acutiloba Kitagawa var. acutiloba Kitagawa, A. acutiloba Kitagawa var. iwatensis Hikino and A. acutiloba Kitagawa var. sugiyamae Hikino have been used as the herbal medicine known in Japan as Japanese Angelica Root. The respective morphological features of, in particular, A. acutiloba var. sugiyamae and A. acutiloba var. iwatensis are similar, and they are not easy to distinguish morphologically from each other. In an attempt to find a method for discriminating among these three varieties, we compared the nucleotide sequence of the spacer region between the atpF and atpA genes among the respective plastid genomes. Comparison of these sequences allowed us to identify each of the three varieties unequivocally.

摘要

北川当归变种北川当归、北川当归变种岩手当归和北川当归变种杉山当归的干燥根在日本被用作草药,称为日本当归根。特别是,杉山当归变种和岩手当归变种各自的形态特征相似,在形态上彼此不易区分。为了找到区分这三个变种的方法,我们比较了各个质体基因组中atpF和atpA基因之间间隔区的核苷酸序列。通过比较这些序列,我们能够明确鉴定出这三个变种中的每一个。

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Population genetic structure of wild Angelica acutiloba, A. acutiloba var. iwatensis, and their hybrids by atpF-atpA intergenic spacer in chloroplast DNA and genome-wide SNP analysis using MIG-seq.利用 MIG-seq 通过叶绿体 DNA 的 atpF-atpA 基因间隔区和全基因组 SNP 分析对野生当归、华当归及其杂种的群体遗传结构进行研究。
J Nat Med. 2023 Sep;77(4):1009-1021. doi: 10.1007/s11418-023-01742-6. Epub 2023 Aug 15.
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