Henn H-J, Wingender R, Schnabl H
Institut für Landwirtschaftliche Botanik der Universität Bonn, Meckenheimer Allee 176, D-53115 Bonn, Germany Fax: +49-228-695168, , , , , , DE.
Plant Cell Rep. 1998 Dec;18(3-4):288-291. doi: 10.1007/s002990050573.
Interspecific hybridisation in the genus Helianthus via somatic cell fusion is thought to play an important role in future sunflower breeding programs. The establishment of this technique requires, however, the development of single-cell-regeneration protocols. For this purpose, we applied a regeneration protocol recently developed for Helianthus annuus L. to mesophyll protoplasts of two wild sunflowers (H. nuttallii T&G, H. giganteus L). Protoplasts of both species were embedded in agarose droplets and covered by liquid mKM medium. After 4-5 weeks, callus was transferred onto solid differentiation medium yielding plating efficencies of 1.5% (H. nuttallii) and 2.5% (H. giganteus). Emerging shoots were elongated on hormone-free medium, and root formation was induced by an NAA treatment. Regenerated plants were transferred to the greenhouse where they grew up to a height of 2 m and flowered after 3 months. Seeds were harvested from regenerated plants of both species.
通过体细胞融合在向日葵属中进行种间杂交被认为在未来的向日葵育种计划中起着重要作用。然而，这项技术的建立需要开发单细胞再生方案。为此，我们将最近为向日葵（Helianthus annuus L.）开发的再生方案应用于两种野生向日葵（H. nuttallii T&G、H. giganteus L）的叶肉原生质体。两种物种的原生质体都包埋在琼脂糖滴中，并用液体mKM培养基覆盖。4-5周后，愈伤组织转移到固体分化培养基上，平板效率分别为1.5%（H. nuttallii）和2.5%（H. giganteus）。长出的芽在无激素培养基上伸长，通过NAA处理诱导生根。再生植株转移到温室中，它们长到2米高，并在3个月后开花。从两种物种的再生植株上收获种子。
