Institut de Biologie Moléculaire des Plantes, CNRS, Université Louis Pasteur, 12, rue du Général Zimmer, 67084, Strasbourg-Cedex, France.
Plant Cell Rep. 1992 Nov;11(12):632-6. doi: 10.1007/BF00236388.
Cotyledon and hypocotyl protoplasts of Helianthus annuus inbred line 47 302 bcd were embedded in alginate and plated on L4 medium (Lenée and Chupeau 1986). After one month, the calli were transferred on MSSH regeneration medium (Murashige and Skoog 1962; Schenk and Hildebrandt 1972) where they regenerated shoots (overall efficiency 10(-2)%). The shoots were elongated on B5 (Gamborg et al. 1968) medium first without hormones, then supplemented with GA3 and BAP (both 0.05 mg/l). In order to overcome the difficulty to induce rooting by classical methods, the elongated shoots were grafted on a sunflower rootstock. The grafted shoots produced flowers and seeds. Different factors have been shown to have an important influence on the capacity to regenerate shoots: the genotype, the physical culture conditions at the callus regeneration step (e.g. protoplasts embedded in alginate), and the media composition.
向日葵自交系 47302bcd 的子叶和下胚轴原生质体嵌入藻酸盐中，并接种在 L4 培养基上（Lenée 和 Chupeau，1986）。一个月后，将愈伤组织转移到 MSSH 再生培养基（Murashige 和 Skoog，1962；Schenk 和 Hildebrandt，1972）上，在那里它们再生出芽（总效率为 10（-2）%）。芽首先在没有激素的 B5（Gamborg 等人，1968）培养基上伸长，然后补充 GA3 和 BAP（均为 0.05 mg/l）。为了克服经典方法诱导生根的困难，将伸长的芽嫁接到向日葵砧木上。嫁接的芽长出花和种子。已经证明，不同的因素对再生芽的能力有重要影响：基因型、愈伤组织再生步骤的物理培养条件（例如，嵌入藻酸盐的原生质体）和培养基组成。
