Shen Xiaoyun, Chi Yongkuan, Huo Bin, Xiong Kangning
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China.
State Engineering Technology Institute for Karst Desertification Control, Guizhou Normal University, Guiyang 550025, China.
Asian-Australas J Anim Sci. 2019 Jun;32(6):896-903. doi: 10.5713/ajas.18.0622. Epub 2019 Jan 2.
Qianbei-Pockmarked goats are affected by a disorder locally referred to as 'Ruanguzheng Disorder', which is characterized by emaciation, lameness, muscular relaxation, stiffness of the extremities, and abnormal curvatures of the long bones. Our objective was to determine the relationship between the disorder and phosphorus deficiency.
Tissue samples were collected from affected and healthy animals, while soil and herbage samples were collected from affected and healthy pastures. Biochemical parameters were determined using an automatic biochemical analyzer (OLYMPUS AU 640, Olympus Optical Co., Tokyo, Japan). Mineral contents in soil, forage, and tissue were determined using a Perkin-Elmer AAS5000 atomic absorption spectrophotometer (Perkin-Elmer, Norwalk, CT, USA).
The results showed that phosphorus contents in herbages from affected pastures were markedly lower than those from healthy areas (p<0.01), and the ratio of calcium to phosphorus in the affected herbages was 12.93:1. The phosphorus contents of wool, blood, tooth, and bone from affected animals were also markedly lower than those from healthy animals (p<0.01). Serum phosphorus values in affected animals were much lower than those in healthy animals, while serum alkaline phosphatase values from affected animals were markedly higher than those from healthy animals (p<0.01). Inorganic phosphorus values from affected animals were approximately half of that in the control group. Supplementation of disodium hydrogen phosphate prevented and cured the disorder.
This study demonstrates that Ruanguzheng disorder in Qianbei-Pockmarked goats is primarily caused by phosphorus deficiencies in herbage due to fenced pastures and natural habitat fragmentation.
黔北麻羊受一种当地称为“软骨症”的疾病影响，其特征为消瘦、跛行、肌肉松弛、四肢僵硬以及长骨异常弯曲。我们的目的是确定该疾病与磷缺乏之间的关系。
从患病和健康动物身上采集组织样本，同时从患病和健康牧场采集土壤和牧草样本。使用自动生化分析仪（OLYMPUS AU 640，日本东京奥林巴斯光学株式会社）测定生化参数。使用珀金埃尔默AAS5000原子吸收分光光度计（美国康涅狄格州诺沃克市珀金埃尔默公司）测定土壤、饲料和组织中的矿物质含量。
结果表明，患病牧场牧草中的磷含量显著低于健康地区（p<0.01），患病牧草中钙磷比为12.93:1。患病动物的羊毛、血液、牙齿和骨骼中的磷含量也显著低于健康动物（p<0.01）。患病动物的血清磷值远低于健康动物，而患病动物的血清碱性磷酸酶值显著高于健康动物（p<0.01）。患病动物的无机磷值约为对照组的一半。补充磷酸氢二钠可预防和治愈该疾病。
本研究表明，黔北麻羊的软骨症主要是由于围栏牧场和自然栖息地破碎化导致牧草中磷缺乏所致。
