School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
State Engineering Technology Institute for Karst Desertification Control, Guizhou Normal University, Guiyang, 550025, China.
Biol Trace Elem Res. 2021 Jun;199(6):2201-2207. doi: 10.1007/s12011-020-02342-1. Epub 2020 Aug 18.
Guizhou black goats are essential to the production system in the Wumeng prairie in the Western China. This study aimed to determine the influence of nano-copper on antioxidant system in copper-deprived Guizhou black goats. We analyzed mineral contents in soil, forage, and goats' tissues. Blood parameters were also determined. The results showed that copper concentrations in soil and forage were significantly lower, and the iron content was significantly higher in affected compared with healthy area (P < 0.01). Copper concentrations in animal tissues (blood, liver, and hair) were significantly lower and iron content was significantly higher in affected compared with healthy goats (P < 0.01). After supplementation of nano-copper or copper sulfate, copper concentration in blood was significantly increased and iron content was significantly lower (P < 0.01). Compared with nano-copper group, the effect of copper sulfate was slower. Hemoglobin levels, erythrocyte count, and packed cell volume from nano-copper and copper sulfate groups were significantly higher than those in copper-deprived goats (P < 0.01). Compared with the copper-deprived Guizhou black goats, serum ceruloplasmin levels in nano-copper and copper sulfate groups were significantly increased, while serum lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and creatinine were significantly decreased (P < 0.01). Compared with the copper-deprived animals, serum superoxide dismutase, glutathione peroxidase, catalase, and total antioxidant capacity in nano-copper and copper sulfate groups were significantly higher, while serum malondialdehyde content was significantly lower (P < 0.01). The effect of copper sulfate group was significantly lower than that in nano-copper group (P < 0.01). Consequently, nano-copper could not only markedly increase the copper content in blood in copper-deprived Guizhou black goats but also much improves the antioxidant capacity.
贵州黑山羊是中国西部乌蒙草原生产系统的重要组成部分。本研究旨在探讨纳米铜对缺铜贵州黑山羊抗氧化系统的影响。我们分析了土壤、饲料和山羊组织中的矿物质含量，同时还检测了血液参数。结果表明，与健康区相比，受影响地区的土壤和饲料中的铜浓度显著降低，铁含量显著升高（P<0.01）。与健康山羊相比，受影响地区动物组织（血液、肝脏和毛发）中的铜浓度显著降低，铁含量显著升高（P<0.01）。补充纳米铜或硫酸铜后，血液中的铜浓度显著升高，铁含量显著降低（P<0.01）。与纳米铜组相比，硫酸铜的效果较慢。纳米铜和硫酸铜组的血红蛋白水平、红细胞计数和红细胞压积均显著高于缺铜组（P<0.01）。与缺铜贵州黑山羊相比，纳米铜和硫酸铜组的血清铜蓝蛋白水平显著升高，而血清乳酸脱氢酶、天门冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶和肌酐水平显著降低（P<0.01）。与缺铜动物相比，纳米铜和硫酸铜组的血清超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶和总抗氧化能力显著升高，而血清丙二醛含量显著降低（P<0.01）。硫酸铜组的效果明显低于纳米铜组（P<0.01）。因此，纳米铜不仅能显著提高缺铜贵州黑山羊血液中的铜含量，还能显著提高其抗氧化能力。
