1 Department of Otolaryngology-Head and Neck Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.
2 Department of Cancer Biology, Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, Pennsylvania, USA.
Otolaryngol Head Neck Surg. 2019 Jul;161(1):67-73. doi: 10.1177/0194599819829741. Epub 2019 Feb 12.
The purpose of this study is to assess CD169 expression in metastatic and nearby tumor-free lymph nodes of patients with head and neck squamous cell carcinoma (SCC).
Retrospective analysis based on immunohistochemistry.
Tertiary care center.
The abundance of CD169 cells in the subcapsular sinuses (SCSs) of lymph nodes was assessed immunohistochemically in paraffin-embedded tissue samples derived from 22 patients with oral cavity and oropharyngeal SCC.
SCSs of lymph nodes harboring metastatic SCC contained significantly fewer CD169 macrophages (106.5 ± 113.6 cells/mm) compared to nearby tumor-free lymph nodes (321.3 ± 173.4 cells/mm, < .001). This observation extended to 21 of the 22 cases investigated. In addition, 6 patients who later developed recurrent disease contained lower numbers of CD169 cells (268.6 ± 169.5 cells/mm) in nearby tumor-free lymph nodes compared to 341.0 ± 176.1 cells/mm in those who remained disease free ( = .399). Human papillomavirus (HPV)-positive patients (n = 4) had a 6-fold lower number of CD169 cells in metastatic nodes (61.2 ± 85.5 cells/mm) compared to nearby tumor-free lymph nodes (369.5 ± 175.5 cells/mm, = .028). In comparison, HPV-negative patients had only a 3-fold reduction (116.6 ± 118.5 cells/mm vs 310.6 ± 176.2 cells/mm, < .001).
Metastatic spread of SCC to regional lymph nodes is associated with lower abundance of CD169 macrophages in the SCSs of draining lymph nodes. These results set the stage for an in-depth investigation into the mechanism(s) by which metastatic SCC controls CD169 macrophage abundance and its significance as it relates to prognosis and treatment response.
本研究旨在评估头颈部鳞状细胞癌（SCC）患者转移和附近无肿瘤淋巴结中 CD169 的表达。
基于免疫组织化学的回顾性分析。
三级保健中心。
对 22 例口腔和口咽 SCC 患者石蜡包埋组织样本的淋巴结被膜下窦（SCS）中 CD169 细胞的丰度进行免疫组织化学评估。
与附近无肿瘤淋巴结（321.3±173.4 个细胞/mm， <.001）相比，含有转移性 SCC 的淋巴结 SCS 中 CD169 巨噬细胞明显减少（106.5±113.6 个细胞/mm）。这一观察结果扩展到 22 例研究中的 21 例。此外，6 例随后发生复发的患者在附近无肿瘤淋巴结中 CD169 细胞数量较低（268.6±169.5 个细胞/mm），而在无疾病患者中为 341.0±176.1 个细胞/mm（ =.399）。人乳头瘤病毒（HPV）阳性患者（n=4）的转移性淋巴结中 CD169 细胞数量减少 6 倍（61.2±85.5 个细胞/mm），而附近无肿瘤淋巴结中 CD169 细胞数量减少 3 倍（369.5±175.5 个细胞/mm， =.028）。相比之下，HPV 阴性患者仅减少 3 倍（116.6±118.5 个细胞/mm 与 310.6±176.2 个细胞/mm， <.001）。
SCC 向区域淋巴结的转移与引流淋巴结 SCS 中 CD169 巨噬细胞丰度降低有关。这些结果为深入研究转移性 SCC 控制 CD169 巨噬细胞丰度的机制及其与预后和治疗反应的关系奠定了基础。
