Biomolecules Lab, Department of Biosciences, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, India.
Department of Biotechnology, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, India.
Microbiol Res. 2019 Mar;220:42-52. doi: 10.1016/j.micres.2018.12.004. Epub 2018 Dec 21.
Contamination of soil by textile effluent is a major threat found worldwide. These pollutants have diverse range of negative effects on the ecosystem, therefore restoration through cost effective biological strategy is the need of the hour. The aim of the current study was to enhance the decolourization of reactive green dye (RGD) using phytoremediation coupled with augmentation of effective bacteria to the rhizosphere. The isolate Klebsiella sp. VITAJ23 was isolated from textile effluent polluted soil and was assessed for its plant growth promoting traits (PGP) and the PGP functional genes were amplified. The soil was artificially polluted with RGD concentration ranging from 1000 to 3000 mg kg and Alternanthera philoxeroides plantlets were planted in phyto and rhizoremediation treatments, the setup was maintained upto 60 d. The isolate VITAJ23 was augmented in the rhizoremediation setup and the morphological parameters were assessed at regular interval. There was a significant increase in the chlorophyll content as well as root and shoot length of the plant when treated with the bacterial suspension. Decolourization study revealed 79% removal of reactive green dye with an enhanced oxido-reductase enzyme activity in the setup bioaugmented with bacteria. The biodegraded metabolites were identified as 2-allylnapthalene, l-alanine, n-acetyl-and propenoic acid by GC-MS analysis and a plant-bacteria degradation pathway was predicted using computational tools. Inoculation of PGP-Klebsiella sp. VITAJ23 enhanced the rate of plant growth and dye degradation.
纺织废水对土壤的污染是全球普遍存在的主要威胁。这些污染物对生态系统有多种负面影响，因此通过具有成本效益的生物策略进行修复是当前的迫切需求。本研究旨在通过植物修复与向根际添加有效细菌相结合来增强活性绿染料（RGD）的脱色效果。从受纺织废水污染的土壤中分离出 Klebsiella sp. VITAJ23，并评估其植物促生特性（PGP）和 PGP 功能基因的扩增情况。土壤中人工添加 RGD 浓度为 1000 至 3000 mg/kg，将Alternanthera philoxeroides 幼苗种植在植物和根际修复处理中，维持 60 天。在根际修复装置中添加了 VITAJ23 分离株，并定期评估形态参数。当用细菌悬浮液处理时，植物的叶绿素含量以及根和茎的长度都有显著增加。脱色研究表明，在添加细菌的生物增强装置中，活性绿染料的去除率达到 79%，同时氧化还原酶活性增强。通过 GC-MS 分析鉴定了生物降解的代谢产物为 2-丙烯基萘、l-丙氨酸、n-乙酰基和丙烯酸，并使用计算工具预测了植物-细菌降解途径。接种 PGP-Klebsiella sp. VITAJ23 可提高植物生长和染料降解速度。
