Institute of Biomedical Materials and Engineering, College of Chemistry and Chemical Engineering, College of Materials Science and Engineering, Qingdao University, Qingdao, 266071, China; Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, Qingdao University, Qingdao, 266071, China.
Institute of Biomedical Materials and Engineering, College of Chemistry and Chemical Engineering, College of Materials Science and Engineering, Qingdao University, Qingdao, 266071, China.
J Chromatogr A. 2019 May 24;1593:174-182. doi: 10.1016/j.chroma.2019.02.004. Epub 2019 Feb 4.
In this paper, we have developed a novel method for the preparation of covalently connected capillary coatings in which diazotized poly (vinyl alcohol-b-styrene) (diazo-P(VA-b-St)) was used as a photosensitive coating agent. Firstly, the diazo-P(VA-b-St) coating was self-assembled on the inner surface of the capillary, and then irradiated by ultraviolet (UV) light to convert the ionic bonding into covalent bonding through the unique photochemical reaction of diazo groups. The covalently connected coatings inhibited the protein adsorption on the inner surface of the capillary, as a result, the baseline protein separation of ribonuclease A (RNase A), lysozyme (Lyz) and bovine serum albumin (BSA) were attained by utilizing the capillary electrophoresis (CE). The covalently connected diazo-P(VA-b-St) capillary coatings have greater CE separation performance with magnificent repeatability and enhanced stability, when compared with non-covalently coated or bare capillaries. This strategy to synthesize photosensitive diazo-P(VA-b-St) capillary coatings for their use in capillary electrophoresis separation of proteins is highly environment-friendly as it does not involve the use of extremely noxious and moisture penetrating coatings of silane.
本文开发了一种新的方法用于制备共价键连接的毛细管涂层，其中重氮聚（乙烯醇-苯乙烯）（diazo-P(VA-b-St)）被用作光敏涂层剂。首先，将 diazo-P(VA-b-St) 涂层自组装在内径毛细管的内表面上，然后通过紫外 (UV) 光照射，通过重氮基团的独特光化学反应将离子键转化为共价键。共价键连接的涂层抑制了蛋白质在内径毛细管内表面的吸附，因此，通过毛细管电泳 (CE) 实现了核糖核酸酶 A (RNase A)、溶菌酶 (Lyz) 和牛血清白蛋白 (BSA) 的基线蛋白分离。与非共价涂覆或裸毛细管相比，共价连接的重氮聚（乙烯醇-苯乙烯）毛细管涂层具有更好的 CE 分离性能、更好的重现性和增强的稳定性。该策略用于合成用于毛细管电泳分离蛋白质的光敏重氮聚（乙烯醇-苯乙烯）毛细管涂层是高度环保的，因为它不涉及使用极其有毒和透湿性的硅烷涂层。
