Yu Bing, Chen Xin, Cong Hailin, Shu Xi, Peng Qiaohong
College of Materials Science and Engineering, Qingdao University, Qingdao, Shandong, 266071, China.
Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, Qingdao, Shandong, 266071, China.
Anal Bioanal Chem. 2016 Sep;408(24):6781-8. doi: 10.1007/s00216-016-9804-5. Epub 2016 Jul 30.
A new method for the fabrication of covalently cross-linked capillary coatings of poly(ethylene glycol) (PEG) is described using diazotized PEG (diazo-PEG) as a new photosensitive coating agent. The film of diazo-PEG depends on ionic bonding and was first prepared on the inner surface of capillary by self-assembly, and ionic bonding was converted into covalent bonding after reaction of ultraviolet light with diazo groups through unique photochemical reaction. The covalently bonded coating impedance adsorption of protein on the central surface of capillary and hence the four proteins ribonuclease A, cytochrome c, bovine serum albumin, and lysosome can be baseline separated by using capillary electrophoresis (CE). The covalently cross-linked diazo-PEG capillary column coatings not only improved the CE separation performance for proteins compared to non-covalently cross-linked coatings or bare capillary but also showed a remarkable chemical solidity and repeatability. Because photosensitive diazo-PEG took the place of the highly noxious and silane moisture-sensitive coating reagents in the fabrication of covalent coating, this technique shows the advantage of being environment-friendly and having a high efficiency for CE to make the covalently bonded capillaries.
本文描述了一种制备聚乙二醇（PEG）共价交联毛细管涂层的新方法，该方法使用重氮化聚乙二醇（重氮-PEG）作为一种新型光敏涂层剂。重氮-PEG薄膜依赖离子键，首先通过自组装在毛细管内表面制备，然后通过独特的光化学反应，紫外线与重氮基团反应后，离子键转化为共价键。共价键合涂层能抑制蛋白质在毛细管中心表面的吸附，因此，使用毛细管电泳（CE）可对核糖核酸酶A、细胞色素c、牛血清白蛋白和溶菌酶这四种蛋白质进行基线分离。与非共价交联涂层或裸毛细管相比，共价交联的重氮-PEG毛细管柱涂层不仅提高了蛋白质的CE分离性能，还表现出显著的化学稳定性和重复性。由于在共价涂层制备中，光敏重氮-PEG取代了高毒性且对湿度敏感的硅烷涂层试剂，该技术显示出环境友好且高效制备共价键合毛细管用于CE的优势。
