Xing Jie, Wang Fang, Cong Hailin, Wang Song, Shen Youqing, Yu Bing
Institute of Biomedical Materials and Engineering, College of Chemistry and Chemical Engineering, College of Materials Science and Engineering, Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266071, China.
Analyst. 2021 Feb 21;146(4):1320-1325. doi: 10.1039/d0an02018d. Epub 2020 Dec 24.
Vancomycin is an amphoteric glycopeptide molecule, and its group diversity and chiral active sites provide a potential basis for its application in chromatographic analysis. In this article, using photosensitive diazo resin (DR) as the coupling agent, vancomycin is modified on the inner wall of the capillary to construct a capillary coating separation system. The highlight of the coated capillary is that it has both anti-protein adsorption and chiral separation properties. Compared with the bare capillary or non-covalently bonded DR/vancomycin-coated capillary, it can not only achieve the separation of four mixed proteins of lysozyme (Lys), bovine serum albumin (BSA), myoglobin (Mb), and ribonuclease A (RNase A), but also shows excellent performance in chiral drugs. The coated capillary effectively solves the problems of low efficiency of the separation column and high sample loss and provides ideas for the development of coated capillaries in the future.
万古霉素是一种两性糖肽分子，其基团多样性和手性活性位点为其在色谱分析中的应用提供了潜在基础。在本文中，以光敏重氮树脂（DR）作为偶联剂，将万古霉素修饰在毛细管内壁上，构建了毛细管涂层分离系统。涂层毛细管的亮点在于它兼具抗蛋白质吸附和手性分离特性。与裸毛细管或非共价键合的DR/万古霉素涂层毛细管相比，它不仅能够实现溶菌酶（Lys）、牛血清白蛋白（BSA）、肌红蛋白（Mb）和核糖核酸酶A（RNase A）四种混合蛋白质的分离，而且在手性药物方面也表现出优异性能。该涂层毛细管有效解决了分离柱效率低和样品损失高的问题，并为未来涂层毛细管的发展提供了思路。
