inhibits growth and metastasis in colon cancer by down-regulating SMAD4.

MicroRNAs (MiRs) are thought to display regulator action in tumor suppression and oncogenesis. plays an important role in the development of various cancers, such as colorectal cancer, breast cancer, and lung cancer, by targetting different molecules potentially involved in many signaling pathways. SMAD4 is a common signaling during tumor progression, and it can inhibit cell proliferation and promote cell motility in most epithelial cells. The present study focused on the effect of and SMAD4 on colon cancer in order to find the novel gene therapy target for the treatment of colon cancer. Quantitative real-time polymerase chain reaction was used to assess the expression level of in colon cancer tissues and SW620 cells. MTT assay, scratch test, and transwell assay were used to evaluate cell proliferation, migration, and invasion, respectively. Moreover, luciferase assays were utilized to identify the predictive effect of on SMAD4. Western blotting was performed to determine the relative expression of protein related to SMAD4. We found level was significantly lower in colon cancer tissues and SW620 cells. Moreover, SMAD4 level, both in mRNA and protein, was obviously elevated in colon cancer tissues. Further, mimics treatment inhibited cells proliferation, invasion, and migration. Fluorescence intensity of mimics group in wild type cells was decreased. mimics repressed the SMAD4 expression both in mRNA and protein. These findings about /SMAD4 pair provide a novel therapeutic method for colon cancer patients.