EBV miR-BART10-3p 通过靶向 DKK1 促进细胞增殖和迁移。
EBV miR-BART10-3p Promotes Cell Proliferation and Migration by Targeting DKK1.
机构信息
Department of Biomedicine & Health Sciences, Department of Medical Lifescience, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
出版信息
Int J Biol Sci. 2019 Jan 24;15(3):657-667. doi: 10.7150/ijbs.30099. eCollection 2019.
In Epstein-Barr virus (EBV)-infected epithelial cancers, HI A rightward transcript (BART) miRNAs are highly expressed. However, only a few target genes of BART miRNAs have been investigated. Our mRNA microarray data showed that DKK1 was markedly down-regulated in EBV-associated gastric carcinoma (EBVaGC) cells. Using luciferase reporter assay we tested whether miR-BART10-3p regulates DKK1 by directly targeting the 3'-UTR of DKK1 mRNA. We observed that miR-BART10-3p transfection decreased DKK1 expression, while an LNA inhibitor of miR-BART10-3p (LNA-miR-BART10-3p(i)) increased DKK1 expression. Furthermore, miR-BART10-3p and siDKK1 promoted cell proliferation and migration. In contrast, transfecting GC cells with LNA-miR-BART10-3p(i) or DKK1 over expression vector suppressed cell proliferation and migration. Our results suggest that miR-BART10-3p may be involved in the tumor progression of EBVaGC by targeting DKK1.
在 Epstein-Barr 病毒（EBV）感染的上皮性癌中，HI A 右向转录（BART）miRNAs 高度表达。然而，仅研究了 BART miRNAs 的少数几个靶基因。我们的 mRNA 微阵列数据显示，EBV 相关胃癌（EBVaGC）细胞中 DKK1 明显下调。我们通过荧光素酶报告基因检测来测试 miR-BART10-3p 是否通过直接靶向 DKK1 mRNA 的 3'-UTR 来调节 DKK1。我们观察到 miR-BART10-3p 转染降低了 DKK1 的表达，而 miR-BART10-3p 的 LNA 抑制剂（LNA-miR-BART10-3p(i)）则增加了 DKK1 的表达。此外，miR-BART10-3p 和 siDKK1 促进了细胞增殖和迁移。相反，用 LNA-miR-BART10-3p(i)或 DKK1 过表达载体转染 GC 细胞可抑制细胞增殖和迁移。我们的研究结果表明，miR-BART10-3p 可能通过靶向 DKK1 参与 EBVaGC 的肿瘤进展。
Zotero 插件