Huang Xiaomin, Zhao Xuhui, Qi Yujiao, Lan Tian, Wang Ruiling, Liang Shuang, Ma Yuxiu, Di Cuixia, Li Hongling
Gansu University of Chinese Medicine, Lanzhou, China.
Gansu Provincial Hospital, Lanzhou, China.
Biochem Genet. 2025 Feb 4. doi: 10.1007/s10528-025-11033-2.
Epstein-Barr virus (EBV), the first human virus identified with oncogenic properties, encodes a class of microRNAs known as miR-BART (BamHI-A rightward transcript microRNAs). This study investigates the pivotal role of EBV-miR-BART14-3p in the progression of gastric cancer, particularly focusing on its effects on epithelial-mesenchymal transition (EMT), cell proliferation, and migration. EBV-associated gastric cancer (EBVaGC) is distinguished by unique genomic and epigenomic characteristics, with EBV miRNAs significantly influencing tumor biology by regulating gene expression. Our research demonstrates that EBV-miR-BART14-3p facilitates gastric cancer cell migration and invasion by targeting the tumor suppressor gene LACTB, which in turn activates the Phosphoinositide 3-kinase (PI3K)/AKT signaling pathway, a critical driver of EMT. The suppression of LACTB in EBVaGC highlights its crucial role in inhibiting tumor progression. These findings position EBV-miR-BART14-3p as a key player in gastric cancer development and underscore its potential as both a prognostic biomarker and a therapeutic target for EBVaGC.
爱泼斯坦-巴尔病毒（EBV）是首个被鉴定具有致癌特性的人类病毒，它编码一类名为miR-BART（BamHI-A向右转录微小RNA）的微小RNA。本研究调查了EBV-miR-BART14-3p在胃癌进展中的关键作用，尤其关注其对上皮-间质转化（EMT）、细胞增殖和迁移的影响。EBV相关胃癌（EBVaGC）具有独特的基因组和表观基因组特征，EBV微小RNA通过调节基因表达对肿瘤生物学产生显著影响。我们的研究表明，EBV-miR-BART14-3p通过靶向肿瘤抑制基因LACTB促进胃癌细胞的迁移和侵袭，进而激活EMT的关键驱动因子磷脂酰肌醇3激酶（PI3K）/AKT信号通路。EBVaGC中LACTB的抑制突出了其在抑制肿瘤进展中的关键作用。这些发现表明EBV-miR-BART14-3p是胃癌发展中的关键因素，并强调了其作为EBVaGC预后生物标志物和治疗靶点的潜力。
