Aboutorabi Roshanak, Behzadi Ehsan, Sadegh Mohammad Javad, Fatehi Seyed Pooriya, Semsarzadeh Soroosh, Zarrin Yasaman, Kazemi Mohammad, Rafiee Laleh, Mostafavi Fatemeh Sadat
Infertility Laboratory, Beheshti Hospital, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
J Reprod Infertil. 2018 Oct-Dec;19(4):211-218.
According to the literature review, polymorphisms of tumor necrosis factor alpha's (TNF-α) promoter region are probably the genetic risk factors of recurrent pregnancy loss. This study has investigated five single nucleotide polymorphisms in the TNF-α gene's promoter region to evaluate their relationship with recurrent pregnancy loss disorder.
Blood samples were taken from 65 women with recurrent pregnancy loss (Case group) and 65 healthy women with a history of successful pregnancy (Control group). Polymerase chain reaction with high resolution melting (PCR-HRM) analysis was done to determine the promoter region of -308G/A, -850T/C, -238G/A, -1031T/C and -863A/C TNF-α polymorphisms. The data were assessed using logistic regression models. P values less than 0.05 were considered statistically significant.
Significant associations were found between recurrent pregnancy loss and -863C/A (p=0.000), -308G/A (p=0.045), and -238G/A (p=0.034) polymorphisms. TNF-α polymorphisms of -863C and -238G may be susceptible factors of recurrent pregnancy loss cases. The -308G polymorphism has an important role in maintaining pregnancy.
The -863C/A and -238G/A TNF-α polymorphisms are possible genetic risk factors of recurrent pregnancy loss and might be its predictive markers.
根据文献综述，肿瘤坏死因子α（TNF-α）启动子区域的多态性可能是复发性流产的遗传风险因素。本研究调查了TNF-α基因启动子区域的五个单核苷酸多态性，以评估它们与复发性流产疾病的关系。
采集65例复发性流产女性（病例组）和65例有成功妊娠史的健康女性（对照组）的血样。采用聚合酶链反应-高分辨率熔解曲线分析（PCR-HRM）来确定TNF-α基因-308G/A、-850T/C、-238G/A、-1031T/C和-863A/C位点启动子区域的多态性。使用逻辑回归模型评估数据。P值小于0.05被认为具有统计学意义。
发现复发性流产与-863C/A（p=0.000）、-308G/A（p=0.045）和-238G/A（p=0.034）多态性之间存在显著关联。-863C和-238G的TNF-α多态性可能是复发性流产病例的易感因素。-308G多态性在维持妊娠中起重要作用。
-863C/A和-238G/A的TNF-α多态性可能是复发性流产的遗传风险因素，可能是其预测标志物。
