To Masahiro, Su Chen-Yao, Hidaka Kouki, Okudera Toshimitsu, Matsuo Masato
Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, 82 Inaoka-cho, Yokosuka, Kanagawa, 238-8580, Japan.
Department of Dentistry, National Yang Ming University, Sec. 2, Linong Street, Taipei, 112, Taiwan.
Anat Sci Int. 2019 Jun;94(3):238-244. doi: 10.1007/s12565-019-00479-1. Epub 2019 Feb 12.
Several methods have been developed to regenerate lost alveolar bone. Platelet-rich fibrin (PRF) is a useful adjunct for new bone formation in dentistry. To elucidate the effect of advanced PRF (A-PRF) on bone formation, we inserted A-PRF clots in sockets after tooth extraction. Premolars were extracted from beagle dogs, and A-PRF was applied to the socket. New bone formation was assessed using histological and immunofluorescence examinations, and the bone formation ratio was evaluated 14 and 30 days postoperatively. Histological examination revealed newly formed bone filling the sockets up to the center in the A-PRF group at 14 days postoperatively, while thick and regular bone trabeculae were arranged in porous bone after 30 days. Higher expressions of osteocalcin and osteopontin were observed in newly formed bone in the A-PRF group, compared to the control group. The bone formation ratio was also higher in the A-PRF group than in the control group. Thus, A-PRF application may result in enhanced new bone formation and may aid in accelerating bone formation. A-PRF was more rapid than a self-limiting process during induction of bone formation by enhancing osteoblast activity and may be useful for bone formation in clinical medicine.
已经开发出几种方法来再生缺失的牙槽骨。富血小板纤维蛋白（PRF）是牙科领域促进新骨形成的一种有用辅助材料。为了阐明高级PRF（A-PRF）对骨形成的影响，我们在拔牙后将A-PRF凝块植入牙槽窝。从比格犬中拔除前磨牙，并将A-PRF应用于牙槽窝。使用组织学和免疫荧光检查评估新骨形成情况，并在术后14天和30天评估骨形成率。组织学检查显示，术后14天，A-PRF组的牙槽窝内新形成的骨组织填充至中心，而30天后，多孔骨内排列着粗大且规则的骨小梁。与对照组相比，A-PRF组新形成骨中骨钙素和骨桥蛋白的表达更高。A-PRF组的骨形成率也高于对照组。因此，应用A-PRF可能会增强新骨形成，并有助于加速骨形成。A-PRF通过增强成骨细胞活性，在诱导骨形成过程中比自限性过程更快，可能对临床医学中的骨形成有用。
