注射用富血小板纤维蛋白（i-PRF）对微型螺钉辅助快速腭扩展器手术扩弓中新骨形成的影响：一项犬模型研究。

Effect of Injectable platelet-rich fibrin (i-PRF) on new bone formation in surgical expansion with mini-screw assisted rapid palatal expander: A dog model study.

作者信息

Awni Khawla M, Dewachi Zaid, Al-Hyani Osama Hazim

机构信息

Department of Orthodontics, College of Dentistry, College of Veterinary Medicine, University of Mosul, Iraq.

Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Mosul, Iraq.

出版信息

J Orthod Sci. 2023 Mar 18;12:12. doi: 10.4103/jos.jos_56_22. eCollection 2023.

DOI:10.4103/jos.jos_56_22

PMID:37351391

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10282521/

Abstract

OBJECTIVES

The objective of the study is to test whether the local injection of i-PRF may affect osteoblast, blood vessels and new bone formation in surgically expanded mid palatal suture using maxillary skeletal expander.

MATERIALS AND METHODS

Eighteen adult male local breed dogs were divided randomly into three groups: group I (control), includes the expansion with maxillary skeletal expander that was done without corticotomy and i-PRF. Group II, involves the expansion that was done with mid palatal corticotomy but without i-PRF injection. Group III, has the expansion that was done with mid palatal corticotomy and has injection with 2 ml of i-PRF. Each group consisted of 6 dogs which were subdivided into 3 dogs. Three dogs were sacrificed after 15 days and the other 3 dogs were sacrificed after 45 days. The number of osteoblast, blood vessels and new bone formation percentage were statistically analyzed using Sigma plot platform. Mean and standard error, ANOVA and Duncan were performed among the different groups. Values of ≤ 0.05 were considered significant.

RESULTS

After expansion, the i-PRF group demonstrated a considerable increase in the amount of new bone in the mid-palatal suture at 15 and 45 days compared with other 2 groups which were indicated by highest percentages of new bone formation (29.30% of 15 days and 76.55% at 45 days) if compared to control group which were (7.72% at 15 days and 22.30% at 45 days). The corticotomy groups were in between, 15.33% and 46.84% respectively. Moreover the number of osteoblasts was higher in corticotomy with i-PRF group on 15 days and decreased on 45 days than the other two groups, while the blood vessels were highest in this group than the other two groups both on 15 and 45 days.

CONCLUSION

I-PRF enhanced the production of osteoblast, blood vessels, and new bone in the surgically expanded mid palatal suture.

摘要

目的

本研究的目的是测试局部注射富血小板纤维蛋白（i-PRF）是否会影响使用上颌骨扩弓器手术扩展的腭中缝处的成骨细胞、血管和新骨形成。

材料与方法

18只成年雄性本地品种犬随机分为三组：第一组（对照组），采用上颌骨扩弓器进行扩弓，不进行皮质切开术和i-PRF注射。第二组，进行腭中皮质切开术但不注射i-PRF。第三组，进行腭中皮质切开术并注射2毫升i-PRF。每组由6只犬组成，再细分为每组3只犬。15天后处死3只犬，另外3只犬在45天后处死。使用Sigma plot平台对成骨细胞数量、血管数量和新骨形成百分比进行统计分析。在不同组之间进行均值和标准误差、方差分析（ANOVA）和邓肯检验。P值≤0.05被认为具有统计学意义。

结果

扩弓后，与其他两组相比，i-PRF组在15天和45天时腭中缝处的新骨量有显著增加，新骨形成百分比最高（15天时为29.30%，45天时为76.55%），而对照组分别为（15天时为7.72%，45天时为22.30%）。皮质切开术组介于两者之间，分别为15.33%和46.84%。此外，与其他两组相比，i-PRF皮质切开术组在15天时成骨细胞数量较多，在45天时减少，而该组在15天和45天时血管数量均高于其他两组。