CMY-1/MOX 家族 AmpCβ-内酰胺酶 MOX-1、MOX-2 和 MOX-9 分别从 3 种气单胞菌属种独立转移。
CMY-1/MOX-family AmpC β-lactamases MOX-1, MOX-2 and MOX-9 were mobilized independently from three Aeromonas species.
机构信息
Center for Antibiotic Resistance Research, Göteborg, Sweden.
Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg, Göteborg, Sweden.
出版信息
J Antimicrob Chemother. 2019 May 1;74(5):1202-1206. doi: 10.1093/jac/dkz025.
OBJECTIVES
To investigate the origin of CMY-1/MOX-family β-lactamases.
METHODS
Publicly available genome assemblies were screened for CMY-1/MOX genes. The loci of CMY-1/MOX genes were compared with respect to synteny and nucleotide identity, and subjected to phylogenetic analysis.
RESULTS
The chromosomal ampC genes of several Aeromonas species were highly similar to known mobile CMY-1/MOX variants. Annotation and sequence comparison revealed nucleotide identities >98% and conserved syntenies between MOX-1-, MOX-2- and MOX-9-associated mobile sequences and the chromosomal Aeromonas sanarellii, Aeromonas caviae and Aeromonas media ampC loci. Furthermore, the phylogenetic analysis showed that MOX-1, MOX-2 and MOX-9 formed three distinct monophyletic groups with the chromosomal ampC genes of A. sanarellii, A. caviae and A. media, respectively.
CONCLUSIONS
Our findings show that three CMY-1/MOX-family β-lactamases were mobilized independently from three Aeromonas species and hence shine new light on the evolution and emergence of mobile antibiotic resistance genes.
目的
探讨 CMY-1/MOX 家族β-内酰胺酶的起源。
方法
筛选公共基因组序列以寻找 CMY-1/MOX 基因。比较 CMY-1/MOX 基因的基因座在同线性和核苷酸同一性方面的差异，并进行系统发育分析。
结果
几种气单胞菌属物种的染色体 ampC 基因与已知的可移动 CMY-1/MOX 变体高度相似。注释和序列比较显示，MOX-1、MOX-2 和 MOX-9 相关的移动序列与气单胞菌属的 Aeromonas sanarellii、Aeromonas caviae 和 Aeromonas media ampC 基因座之间具有>98%的核苷酸同一性和保守的同线性。此外，系统发育分析表明，MOX-1、MOX-2 和 MOX-9 分别与 Aeromonas sanarellii、Aeromonas caviae 和 Aeromonas media 的染色体 ampC 基因形成了三个独特的单系群。
结论
我们的研究结果表明，三种 CMY-1/MOX 家族β-内酰胺酶分别从三种气单胞菌属物种中独立转移而来，从而为移动抗生素耐药基因的进化和出现提供了新的视角。
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