在莎草（Cyperus iria L.）的细胞悬浮培养物中昆虫保幼激素III及其前体的生产

Production of insect juvenile hormone III and its precursors in cell suspension cultures of the sedge, Cyperus iria L.

作者信息

Bede J C, Teal P, Tobe S S

机构信息

Department of Zoology, University of Toronto, 25 Harbord St., Toronto, Ontario, M5S 3G5, Canada e-mail:

CMAVE, United States Department of Agriculture, ARS, Gainesville, FL, P.O. Box 14565, 1700 SW 23 Dr1. Gainesville, FL 32604, USA, , , , , , US.

出版信息

Plant Cell Rep. 1999 Nov;19(1):20-25. doi: 10.1007/s002990050704.

DOI:10.1007/s002990050704

PMID:30754753

Abstract

Juvenile hormones (JHs) are sesquiterpenoids that regulate metamorphosis and reproduction in most insect species. There has been one report of an insect JH in plants: JH III, methyl-10R,11-epoxy-3,7,11-trimethyl 2E, 6E-dodecadienoate, has been identified in two sedge species, Cyperus iria L. and C. aromaticus (Ridley) Mattf and Kük. This is the first report of callus and cell suspension cultures derived from C. iria. Farnesol and methyl farnesoate, two biosynthetic intermediates of JH III in insects, as well as JH III have been identified in suspension culture cell extracts by gas chromatography-mass spectroscopy. These cultures thus provide a useful in vitro model to investigate the biosynthesis of JH III in the sedge, C. iria.

摘要

保幼激素（JHs）是倍半萜类化合物，可调节大多数昆虫物种的变态和繁殖。有一份关于植物中昆虫保幼激素的报告：保幼激素III，即10R,11-环氧-3,7,11-三甲基-2E,6E-十二碳二烯酸甲酯，已在两种莎草科植物，即碎米莎草（Cyperus iria L.）和香附子（C. aromaticus (Ridley) Mattf and Kük）中被鉴定出来。这是关于源自碎米莎草的愈伤组织和细胞悬浮培养物的首次报告。法尼醇和法尼酸甲酯是昆虫中保幼激素III的两种生物合成中间体，通过气相色谱-质谱联用技术在悬浮培养细胞提取物中鉴定出了它们以及保幼激素III。因此，这些培养物为研究莎草科植物碎米莎草中保幼激素III的生物合成提供了一个有用的体外模型。