Moriwaki M, Yamakawa T, Washino T, Kodama T, Igarashi Y
Department of Biotechnology, The University of Tokyo, Yayoi, 1-chome, Bunkyo-ku, Tokyo 113-8657, Japan, , , , , , JP.
Research and Development Section, San-Ei Gen F.F.I., Inc., 1-11, Sanwa-cho-1-chome, Toyonaka City, Osaka 561-8588, Japan, , , , , , JP.
Plant Cell Rep. 1999 Nov;19(1):96-100. doi: 10.1007/s002990050717.
The expression of the Arabidopsis heat shock protein (HSP) 18.2 promoter-β-D-glucuronidase (GUS) chimera gene was investigated in transgenic Nicotiana plumbaginifolia plants during the recovery phase at normal temperatures (20-22 °C) after a heat shock (HS) treatment. GUS activity increased during the recovery phase after HS at 42 °C for 2 h, and maximal GUS activity was observed after 12 h at normal temperatures, at levels 50-100 times higher than the activity immediately after HS. After HS at 44 °C, little GUS activity was observed during the first 20-24 h at normal temperatures, but the activity increased gradually thereafter, to reach a maximum at 40-50 h. After HS at 45 °C, no GUS activity was observed throughout the experimental period. RT-PCR analysis showed that GUS mRNA remained for 10 h after a 2-h HS at 42 °C and for 40 h after a 2-h HS at 44 °C. These findings demonstrate that brief HS treatment, especially at a sublethal temperature, induces a long-term accumulation of HSP-GUS mRNA during the recovery phase.
在热激（HS）处理后的常温（20-22°C）恢复阶段，对转基因矮牵牛植株中拟南芥热激蛋白（HSP）18.2启动子-β-D-葡萄糖醛酸酶（GUS）嵌合基因的表达进行了研究。在42°C热激2小时后恢复阶段，GUS活性增加，在常温下12小时后观察到最大GUS活性，其水平比热激后立即测定的活性高50-100倍。在44°C热激后，常温下最初20-24小时几乎观察不到GUS活性，但此后活性逐渐增加，在40-50小时达到最大值。在45°C热激后，在整个实验期间均未观察到GUS活性。逆转录-聚合酶链反应（RT-PCR）分析表明，在42°C热激2小时后，GUS mRNA保留10小时，在44°C热激2小时后保留40小时。这些结果表明，短暂的热激处理，尤其是在亚致死温度下，会在恢复阶段诱导HSP-GUS mRNA的长期积累。
