Kiviharju E, Puolimatka M, Saastamoinen M, Pehu E
Agricultural Research Centre of Finland, Plant Production Research, Crops and Soil, FIN-31600 Jokioinen, Finland e-mail:
Boreal Plant Breeding, FIN-31600 Jokioinen, Finland, , , , , , FI.
Plant Cell Rep. 2000 Jun;19(7):674-679. doi: 10.1007/s002999900165.
To improve plant regeneration from oat anther culture, the basic medium, hormonal supplements and genotype effect were studied. Six of the 14 genotypes tested regenerated plants. Cultivars Kolbu, Katri, Stout and naked oat Lisbeth produced green plants, cultivars Virma and line OT 257 only albinos. The total number of green plantlets regenerated was 22, of which 13 (11 haploid, 2 doubled haploid) survived into the greenhouse, and 37 albinos. Regenerable-type embryos were induced from heat-pretreated anthers on media containing 2, 3 or 5 mg l 2,4-dichlorophenoxyacetic acid and 0.2 or 0.5 mg l kinetin as hormonal supplements. 6-Benzylaminopurine promoted albino plant regeneration especially in W medium. Colchicine treatment was applied successfully to haploid regenerants.
为提高燕麦花药培养的植株再生能力，对基本培养基、激素添加物和基因型效应进行了研究。在测试的14个基因型中，有6个再生出了植株。品种Kolbu、Katri、Stout和裸燕麦Lisbeth产生了绿色植株，品种Virma和品系OT 257只产生了白化苗。再生的绿色小植株总数为22株，其中13株（11株单倍体，2株双单倍体）存活到温室中，白化苗有37株。在含有2、3或5 mg/L 2,4-二氯苯氧乙酸和0.2或0.5 mg/L激动素作为激素添加物的培养基上，从经热预处理的花药中诱导出可再生成植株类型的胚。6-苄基腺嘌呤尤其在W培养基中促进白化植株的再生。秋水仙素处理成功应用于单倍体再生植株。
