Warchoł Marzena, Juzoń Katarzyna, Dziurka Kinga, Czyczyło-Mysza Ilona, Kapłoniak Kamila, Marcińska Izabela, Skrzypek Edyta
Department of Biotechnology, The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland.
Plants (Basel). 2021 Jan 28;10(2):248. doi: 10.3390/plants10020248.
Oat ( L.) cultivars 'Bingo' and 'Chwat' were used to compare the embryogenesis competence of another culture. Despite the embryo-like structures obtained from both tested cultivars, only 'Chwat' produced green plantlets, which confirmed the cultivar dependency. 'Chwat' produced the highest number of embryo-like structures and green plantlets (0.7/100 anthers and 0.1/100 anthers, respectively). The embryo-like structure formation also depended on cold pretreatment combined with Cu, Zn, or Ag ion supplementation, which was applied during the tiller pretreatment or added to the induction media. The highest number of embryo-like structures (2.1/100 anthers) were observed on anthers derived from the tillers kept in a 50% Hoagland medium with the addition of 10 µM of CuSO. In turn, the induction media supplemented with the ions Cu, Zn, or Ag increased neither the number of embryo-like structures nor the green plantlet production compared to the control conditions. However, such ion applications turned out to be most effective when the induction medium was enriched with 25 µM of AgNO and left to obtain the highest number of embryo-like structures and green plantlets (0.8/100 anthers and 0.2/100 anthers, respectively). Therefore, more attention should be paid to the possibilities of adjusting the media nutrient composition, as this may be the only way to significantly increase the efficiency of this method.
燕麦（L.）品种“宾果”和“Chwat”被用于比较另一种培养物的胚胎发生能力。尽管从两个受试品种中都获得了胚状结构，但只有“Chwat”产生了绿色小植株，这证实了品种依赖性。“Chwat”产生的胚状结构和绿色小植株数量最多（分别为0.7/100个花药和0.1/100个花药）。胚状结构的形成还取决于冷预处理与铜、锌或银离子补充相结合，这些处理在分蘖预处理期间进行或添加到诱导培养基中。在来自保存在添加了10 µM硫酸铜的50%霍格兰培养基中的分蘖的花药上观察到最多的胚状结构（2.1/100个花药）。与对照条件相比，添加铜、锌或银离子的诱导培养基既没有增加胚状结构的数量，也没有增加绿色小植株的产量。然而，当诱导培养基富含25 µM硝酸银并放置以获得最多的胚状结构和绿色小植株（分别为0.8/100个花药和0.2/100个花药）时，这种离子应用被证明是最有效的。因此，应该更加关注调整培养基营养成分的可能性，因为这可能是显著提高该方法效率的唯一途径。
